Ippk (BC024630) Mouse Tagged ORF Clone

CAT#: MG206804

Ippk (tGFP-tagged) - Mouse inositol 1,3,4,5,6-pentakisphosphate 2-kinase (cDNA clone MGC:28631 IMAGE:4222376)

Product Images

Circular map for MG206804

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ippk
• Synonyms: 1810043M15Rik; InsP6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206804 representing BC024630
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGACTTTTTGGGAGAGAACTATGTTCATTGTGGGGAAGTTGTTCAGCTGCCTTTAGAGTTTGTGA
  AACAACTCTGTTTAAAGATACAGTGTGAAAGACCAGAGTCCCGTTGTGACAAGGACCTGGACACATTCAG
  TGGTTATGCTATGTGCCTTCCCAATTTAACCAGACTTCAGACCTTCCACTTTGCAGAGCACCGGCCGATT
  CTGTGTGTAGAGATTAAGCCAAAATGTGGGTTTATTCCTTTCTCAAATGATGTTACCCACGAGATGAAGC
  ACAAGGTGTGCCGATACTGCATGCACCAGCACCTCAAGGTAGCAACTGGAAAGTGGAAGAAAATCAGTAA
  ATACTGTCCCCTCGACCTCTACTCAGGAAATAAGCAGCGCATGCACTTTGCCCTCAGAAGTCTGCTGCAG
  GAGACACAGAATAATCTTAGGATATTTAAGAATGGAGAGCTGATTTATGGTTGTGGTGATGCCCGGAGCC
  CTGTGGCTGACTTGAAGGAGCTTGCACACCACCTGAAGCCCTTCTTCTTCCCTTCCAACGGCTTGGCCAG
  TGGGCCTCACTGCACGAAGGCTGTGATCCGTGAGCTGGTGCATGTTATCACCAGGGTGCTGCTGAGCAGC
  TCAGAAAAAGCCCGAGCTGGTGCCCTGAGGCTTGGGCTCCAGGGCCCACGAGTCTGTGAAGCCAGTCCTT
  TCAGCAGGAGCCTACACAACCAAGGAAAAAACACCTCAGAGCACTCGGGGTTACCGAAGGGCTGTCTTCT
  GTACAAAACCCTCCAGGTGCAGATGTTGGACCAGCTGGACATCGAAGGCCTCTACCCTCTGTACAAACGG
  GTTGAGCAATACCTGGAGGAGTTTCCTGAGGAGAGGAAAACATTACAGATAGATGGGCCTTATGATGAAG
  TATTTTACCAGAAACTGCTTGATCTGTCCACTGAGGATGATGGGACAGTGGCCTTTGCATTAACAAAGGT
  GCAGCAATACAGAGTAGCCATGACTGCCAAGGACTGTTCCATCATGATCGCACTTTCCCCTTGTCTACAG
  GGCACCAGCTCTGACCAGAGGCCTGTTATTCCTTCGTCAAGATCCCGGCTGGCCTTCTCTGTGTCTGTAT
  TGGACCTCGACCTCAAGCCCTATGAGAGCATTCCTCATCAGTATAAACTAGACAGCAAGATAGTCAACTA
  TTACTCAAAGACTGTGCATGCCAAAGATGACACTGTGAGGTCGACTCGGTTCAAGGAACATGAAGATTGC
  ACATTAGTTCTCCATAAGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206804 representing BC024630
  Red=Cloning site Green=Tags(s)
  
  MKDFLGENYVHCGEVVQLPLEFVKQLCLKIQCERPESRCDKDLDTFSGYAMCLPNLTRLQTFHFAEHRPI
  LCVEIKPKCGFIPFSNDVTHEMKHKVCRYCMHQHLKVATGKWKKISKYCPLDLYSGNKQRMHFALRSLLQ
  ETQNNLRIFKNGELIYGCGDARSPVADLKELAHHLKPFFFPSNGLASGPHCTKAVIRELVHVITRVLLSS
  SEKARAGALRLGLQGPRVCEASPFSRSLHNQGKNTSEHSGLPKGCLLYKTLQVQMLDQLDIEGLYPLYKR
  VEQYLEEFPEERKTLQIDGPYDEVFYQKLLDLSTEDDGTVAFALTKVQQYRVAMTAKDCSIMIALSPCLQ
  GTSSDQRPVIPSSRSRLAFSVSVLDLDLKPYESIPHQYKLDSKIVNYYSKTVHAKDDTVRSTRFKEHEDC
  TLVLHKV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC024630
• ORF Size: 1284 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024630.1
• RefSeq Size: 2911 bp
• RefSeq ORF: 1283 bp
• Locus ID: 75678
• Cytogenetics: 13 A5
• Gene Summary: Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-alpha-induced apoptosis.[UniProtKB/Swiss-Prot Function]