Zfp509 (BC028988) Mouse Tagged ORF Clone

CAT#: MG206568

Zfp509 (tGFP-tagged) - Mouse zinc finger protein 509 (cDNA clone MGC:36529 IMAGE:5376301)

Product Images

Circular map for MG206568

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Zfp509
• Synonyms: 4930518A03Rik; Zfp509; Znf509
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206568 representing BC028988
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTTGGCCAGGAGGGAGGAGTCTGCTGCTAAGGAAGATGCAGTGGAGAGAGCTGGAAGCCAGACTGC
  TGAAGAAAAAGGGAGGGGAGAACTGGGTCCAGAGAGCAGCCGTGAGGAGGAGCTGCCAGGAGCCCCAGCT
  TCCTGGGAAGACCCGTCCCAGGCTCTCCAGCCCCAGAAACAGTATGCATGTGAATTGTGTGGGAAGCCTT
  TTAAACACCCAAGCAATCTGGAGCTGCACAAACGGTCTCATACAGGCAGGCAACCTTCAGACTCACTTAC
  GCCGGCACTCTGGGGAGAAGCCATACATCTGTGAAATCTGTGGAAAGAGGTTTGCGGCCTCTGGTGACGT
  CCAGCGCCACATCATCATTCACTCTGGAGAGAAGCCACACTTGTGTGACACGTGCGGCCGAGGGTTCAGC
  AATTTCAGCAATTTGAAAGAGCACAAAAAGACACACACGGCTGACAAGGTGTTTACCTGTGACGAGTGTG
  GGAAGTCCTTTAACATGCAGAGGAAGCTGGTGAAGCACCGCGTGCGGCACACGGGGGAGCGGCCATACAG
  CTGCCCTGCCTGTGGGAAGTGCTTTGGGGGCTCAGGAGACCTGCGCAGGCACGTGCGCACTCACACTGGG
  GAGAAGCCCTATAGCTGTGAGGTCTGCAGCAAGTGTTTCACACGCTCGGCTGTGTTGCGGCGGCACAAGA
  GGATGCACGGCAGAGCTGATGCCAGGAGCCCCGTTGTGCTGGGTGAGCTGAGCCGGCCCATTGAGCCCTC
  TGACCTGGACAGGTCTCAGAGCTCGGACTCCTTCTCCCAGGATGTGTCTGTCACACTGATGCCTGTGTCT
  GTCAAGCTCCCTGTGCAGCCTGTGGAAAGTTCTGTGGCAGGATTCGATGGCCACTGTAGTGGCTCCTACT
  GTAAGTTACGCTCCATGCTTCGCCCTCCTGGCATGAGTGACCAGGACAGACTGAGCCTGGAACCTTCTAA
  GCTGGCCAAGCCACCGGAGCTGCAGAGCCAGCCACAGGCCTACGCGTACTCAGATGTGGAGCCCTCAGCA
  GGTGTGGAGCAGCCACAGGCCGATGGCATGGCTGTGTCCCGCTCTTCCCTGGCCACTCTGGACAACCACT
  GCACTGAGCCACTGGGCAGCCGGGCACCTTCGGTGACCTACAGGAACTCTGAGGGACAGTTTTTCTCCAG
  TATGACTCTCTGGGGGCTGGCAATGAAGACACTGCAGAATGAACATGAGCTGGAGCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206568 representing BC028988
  Red=Cloning site Green=Tags(s)
  
  MGWPGGRSLLLRKMQWRELEARLLKKKGGENWVQRAAVRRSCQEPQLPGKTRPRLSSPRNSMHVNCVGSL
  LNTQAIWSCTNGLIQAGNLQTHLRRHSGEKPYICEICGKRFAASGDVQRHIIIHSGEKPHLCDTCGRGFS
  NFSNLKEHKKTHTADKVFTCDECGKSFNMQRKLVKHRVRHTGERPYSCPACGKCFGGSGDLRRHVRTHTG
  EKPYSCEVCSKCFTRSAVLRRHKRMHGRADARSPVVLGELSRPIEPSDLDRSQSSDSFSQDVSVTLMPVS
  VKLPVQPVESSVAGFDGHCSGSYCKLRSMLRPPGMSDQDRLSLEPSKLAKPPELQSQPQAYAYSDVEPSA
  GVEQPQADGMAVSRSSLATLDNHCTEPLGSRAPSVTYRNSEGQFFSSMTLWGLAMKTLQNEHELEQ
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC028988
• ORF Size: 1250 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028988, AAH28988
• RefSeq Size: 2736 bp
• RefSeq ORF: 1250 bp
• Locus ID: 75079
• Cytogenetics: 5 B3
• Gene Summary: Transcription factor. Inhibits cell proliferation by activating either CDKN1A/p21 transcription or RB1 transcription.[UniProtKB/Swiss-Prot Function]