Mmaa (NM_133823) Mouse Tagged ORF Clone

CAT#: MG206557

Mmaa (tGFP-tagged) - Mouse methylmalonic aciduria (cobalamin deficiency) type A (Mmaa)

Product Images

Circular map for MG206557

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mmaa
• Synonyms: 2810018E08Rik; AI840684
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206557 representing NM_133823
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTATTTCCACGCTGCTACTTTCTCCTAATCGGCGTTTACTAACCTGCCTCTCAAGAGTGCCTTCCC
  CATGGCTCCTTCACTCAAGTCCCCCTGCACCGGGACCCCCAGGTGCTCTGCCAAACTGTTTTGGACACCA
  TTGTACTAAGAGGGTGCTACTGTCTGACGGCTTCAGGAGAACATTATGTGTCCAGGCAACCTTGAAGGAC
  CACACAGAAGGACTTTCTGATAAAGAACAAAGATTTGTGGATAGACTTTATACGGGTTTAGTGAAAGGAC
  AGAGAGCTTGCCTAGCGGAGGCCATAACTCTCGTGGAATCAACCCATACAAGGAAGAGGGAGCTGGCTCA
  GGTGCTTCTGCAGAGAGTCTTAGCCCTCCAGCGGGAGCAGGAGCTGCGGAACCAAGGAAAGCCCCTCACA
  TTTCGAGTAGGACTGTCCGGGCCCCCTGGTGCAGGAAAATCAACATTCATAGAATGTTTTGGGAAAATGC
  TGACTGAGCAAGGGCACAGGTTATCTGTGCTAGCTGTGGATCCATCTTCTTGCACCAGTGGTGGGTCCCT
  CTTAGGTGATAAAACCCGGATGATTGAGCTGTCAAGAGATATGAATGCCTACATCAGGCCCTCTCCTACC
  AGTGGGACTCTAGGAGGAGTGACAAGGACCACAAATGAAGCCATTGTGTTGTGTGAAGGAGGGGGCTATG
  ACATCATTCTTATTGAAACCGTCGGTGTAGGGCAGTCGGAGTTTGCTGTGGCTGACATGGTCGATATGTT
  TGTTTTATTGCTGCCACCAGCAGGAGGGGATGAACTGCAGGGCATCAAAAGGGGCATCATTGAAATGGCA
  GATTTGGTTGTTATAACTAAATCTGATGGAGACTTGATTGTGCCAGCCCGCAGGATCCAAGCAGAGTACG
  TGAGCGCACTCAAGTTGCTCCGTAGGCGCTCGGAAGTCTGGAGGCCAAAGGTGATTCGCATTTCTGCCAG
  AAGTGGAGAGGGCATCACTGAGATGTGGGACACAATGAGAGAGTTTCAGCACCAAATGCTGGCCAGTGGG
  GAGCTGGCTGCCAAGAGACAGACACAGCACAAAGTCTGGATGTGGAATCTCATTCAGGAAAACGTCCTAG
  AACACTTCAAGACCCACCCCAGCATCCGAGAACAGATCCCCCTGATGGAGAGAAAGGTCCTCAGTGGAGC
  CCTCTCCCCAGGACGAGCAGCAGACTTGTTGCTAAAAGCTTTTAAAAGCAGACAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206557 representing NM_133823
  Red=Cloning site Green=Tags(s)
  
  MTISTLLLSPNRRLLTCLSRVPSPWLLHSSPPAPGPPGALPNCFGHHCTKRVLLSDGFRRTLCVQATLKD
  HTEGLSDKEQRFVDRLYTGLVKGQRACLAEAITLVESTHTRKRELAQVLLQRVLALQREQELRNQGKPLT
  FRVGLSGPPGAGKSTFIECFGKMLTEQGHRLSVLAVDPSSCTSGGSLLGDKTRMIELSRDMNAYIRPSPT
  SGTLGGVTRTTNEAIVLCEGGGYDIILIETVGVGQSEFAVADMVDMFVLLLPPAGGDELQGIKRGIIEMA
  DLVVITKSDGDLIVPARRIQAEYVSALKLLRRRSEVWRPKVIRISARSGEGITEMWDTMREFQHQMLASG
  ELAAKRQTQHKVWMWNLIQENVLEHFKTHPSIREQIPLMERKVLSGALSPGRAADLLLKAFKSRH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133823
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133823.1
• RefSeq Size: 2824 bp
• RefSeq ORF: 1248 bp
• Locus ID: 109136
• UniProt ID: Q8C7H1
• Cytogenetics: 8 C1
• Gene Summary: GTPase, binds and hydrolyzes GTP. Involved in intracellular vitamin B12 metabolism, mediates the transport of cobalamin (Cbl) into mitochondria for the final steps of adenosylcobalamin (AdoCbl) synthesis. Functions as a G-protein chaperone that assists AdoCbl cofactor delivery from MMAB to the methylmalonyl-CoA mutase (MMUT) and reactivation of the enzyme during catalysis.[UniProtKB/Swiss-Prot Function]