Tank (BC026148) Mouse Tagged ORF Clone

CAT#: MG206499

Tank (tGFP-tagged) - Mouse TRAF family member-associated Nf-kappa B activator (cDNA clone MGC:13977 IMAGE:3492937)

Product Images

Circular map for MG206499

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tank
• Synonyms: C86182; E430026L09Rik; I-TRAF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206499 representing BC026148
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAAAAACATTGGTGAGCAACTCAATAGAGCATATGAAGCCTTCCGACAGGCATGCATGGATAGAG
  ATTCAGCAGTAAGAGAGCTACAGCAAAAGACTGAGAACTATGAACAAAGAATACGCGAGCAACAGGAACA
  GCTGTCATTTCAACAAAACCTAATTGACAGGCTGAAATCACAGCTACTTCTCGTGGATTCTAGTCGAGAT
  AACAGTTATGGCTATGTACCTTTGCTTGAAGACAGTGACAGAAGGAAGAATAATTTGACCCTTGATGAAC
  CACATGATAAAGTGAAACTAGGAACACTGAGATATAAGCAATCAAAGGTGAGACGACAAGAAGTTTCTTC
  TGGAAAAGAATCCGCCAAGGGTCTCAACATCCCTCTGCATCACGAAAGGGATAATATAGAGAAGACTTTC
  TGGGACCTTAAAGAAGAATTTCATAGGATTTGCTTGCTAGCAAAAGCACAGAAAGACCACTTAAGCAAAC
  TTAATATACCAGATATTGCAACTGACACACAGTGTTCTGTGCCTATACAGTGTACTGATAAAACAGAGAA
  ACAAGAAGCGCTGTTTAAGCCCCAGGCTAAAGATGATATAAATAGAGGTATGTCGTGCGTCACAGCTGTC
  ACACCAAGAGGACTGGGCCGGGATGAGGAAGATACCTCTTTTGAATCACTTTCTAAATTCAATGTCAAGT
  TTCCGCCTATGGACAATGACTCTATTTTTCTACATAGCACTCCAGAGGCCCCAAGCATCCTTGCTCCTGC
  CACACCTGAGACAGTGTGCCAGGACCGATTTAATATGGAAGTCAGAGACAACCCAGGAAACTTTGTTAAA
  ACAGAAGAAACTTTATTTGAAATTCAGGGAATTGACCCCATAACTTCAGCTATACAAAACCTTAAAACAA
  CTGACAAAACAAACCCCTCAAATCTTAGAGCGCCGTGTTTGCCAGCTGGAGACCACAATGTGTTCTATGT
  AAATACGTTCCCACTTCAAGACCCGCCTGACGCACCTTTTCCCTCACTGGATTCCCCAGGAAAGGCTGTC
  CGAGGACCACAGCAGCCCTTTTGGAAGCCTTTTCTTAACCAAGACACTGACTTAGTGGTACCAAGTGATT
  CAGACTCAGAGCTCCTTAAACCTCTAGTGTGTGAATTCTGTCAAGAGCTTTTCCCACCATCCATTACATC
  CAGAGGGGATTTCCTCCGGCATCTTAATACACACTTTAATGGGGAGACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206499 representing BC026148
  Red=Cloning site Green=Tags(s)
  
  MDKNIGEQLNRAYEAFRQACMDRDSAVRELQQKTENYEQRIREQQEQLSFQQNLIDRLKSQLLLVDSSRD
  NSYGYVPLLEDSDRRKNNLTLDEPHDKVKLGTLRYKQSKVRRQEVSSGKESAKGLNIPLHHERDNIEKTF
  WDLKEEFHRICLLAKAQKDHLSKLNIPDIATDTQCSVPIQCTDKTEKQEALFKPQAKDDINRGMSCVTAV
  TPRGLGRDEEDTSFESLSKFNVKFPPMDNDSIFLHSTPEAPSILAPATPETVCQDRFNMEVRDNPGNFVK
  TEETLFEIQGIDPITSAIQNLKTTDKTNPSNLRAPCLPAGDHNVFYVNTFPLQDPPDAPFPSLDSPGKAV
  RGPQQPFWKPFLNQDTDLVVPSDSDSELLKPLVCEFCQELFPPSITSRGDFLRHLNTHFNGET
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC026148
• ORF Size: 1241 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026148, AAH26148
• RefSeq Size: 2084 bp
• RefSeq ORF: 1241 bp
• Locus ID: 21353
• Cytogenetics: 2 C1.3
• Gene Summary: Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. Negatively regulates NF-kappaB signaling and cell survival upon DNA damage. Plays a role as an adapter to assemble ZC3H12A, USP10 in a deubiquitination complex which plays a negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Promotes UBP10-induced deubiquitination of TRAF6 in response to DNA damage. May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2. Essential for the efficient induction of IRF-dependent transcription following infection with Sendai virus.[UniProtKB/Swiss-Prot Function]