Mvd (NM_138656) Mouse Tagged ORF Clone

CAT#: MG206298

Mvd (tGFP-tagged) - Mouse mevalonate (diphospho) decarboxylase (Mvd)

Product Images

Circular map for MG206298

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mvd
• Synonyms: C78718; Mpd
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206298 representing NM_138656
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCAGAAAAGCCTCAGGACCTAATGGTCACCTGCACGGCGCCGGTCAACATCGCAGTTATCAAGT
  ACTGGGGGAAGCGAGATGAAGCGCTGATCCTGCCCATCAACTCCTCCCTGAGCGTCACGCTGCACCAGGA
  CCAGCTAAAAACGACCACAACCGTTGCCATTAGCAAGGACTTCACAGAGGACCGCATCTGGCTGAACGGT
  CGCGAGGAGGACGTGGGGCAGCCACGGCTCCAGGCCTGCCTGAGAGAGATTCGCCGCCTGGCTCGGAAGC
  GGAGGAGCACAGAGGACGGGGACACTCTACCCCTCAGCCTCAGCTATAAGGTGCACGTGGCGTCAGTGAA
  CAACTTCCCCACTGCTGCAGGCCTGGCGTCCTCAGCCGCAGGCTATGCCTGCCTAGCCTATACCCTGGCT
  CAGGTCTATGGGGTTGAGGGAGACCTCTCCGAAGTGGCTCGGCGGGGCTCAGGCAGTGCGTGCCGCAGTC
  TCTATGGGGGCTTTGTGGAGTGGCAGATGGGGGAACAGGCCGATGGGAAGGACAGCATCGCCCGGCAGAT
  AGCCCCAGAGTGGCACTGGCCCCAGCTCCGAATCCTTATCCTTGTGGTGAGCGCCGACAAGAAGCAGACG
  GGCAGTACAGTGGGCATGCAGACCAGCGTGGAGACCAGCACCCTGCTCAAGTTCCGGGCTGAGTCTGTTG
  TGCCTGAGCGCATGAAGGAGATGACCCGCTGCATCCAAGAGCAGGACTTTCAGGGCTTTGCCCAGCTGAC
  CATGAAGGATAGCAACCAGTTTCATGCCACCTGCCTGGACACCTTCCCACCGATCTCCTACCTCAATGAC
  ACCTCCAGGCGCATCATCCAGCTAGTCCACCGCTTCAACACCCACCACGGGCAGACGAAGGTGGCATACA
  CATTTGATGCTGGCCCCAACGCTGTGATCTTCACTCTGGAGGACACTGTGGCTGAGTTTGTGGCAGCTGT
  AAGACACAGCTTTCCCCCTGCAGCCAATGGAGACAAGTTCCTAAAGGGGCTGCAGGTGGCACCTGTTCTT
  CTCTCTGATGAGCTGAAGGCCGCGCTGGTGGTGGAGCCCAGCCCTGGTGGGGTCCAGTACATCATTGCCA
  CTCAGGTCGGACCAGGACCTCAAGTCCTAGACGACACTCATGATCATCTGCTAGGCCAGGATGGACTGCC
  ACAACGGGACCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206298 representing NM_138656
  Red=Cloning site Green=Tags(s)
  
  MASEKPQDLMVTCTAPVNIAVIKYWGKRDEALILPINSSLSVTLHQDQLKTTTTVAISKDFTEDRIWLNG
  REEDVGQPRLQACLREIRRLARKRRSTEDGDTLPLSLSYKVHVASVNNFPTAAGLASSAAGYACLAYTLA
  QVYGVEGDLSEVARRGSGSACRSLYGGFVEWQMGEQADGKDSIARQIAPEWHWPQLRILILVVSADKKQT
  GSTVGMQTSVETSTLLKFRAESVVPERMKEMTRCIQEQDFQGFAQLTMKDSNQFHATCLDTFPPISYLND
  TSRRIIQLVHRFNTHHGQTKVAYTFDAGPNAVIFTLEDTVAEFVAAVRHSFPPAANGDKFLKGLQVAPVL
  LSDELKAALVVEPSPGGVQYIIATQVGPGPQVLDDTHDHLLGQDGLPQRDL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138656
• ORF Size: 1203 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138656.1, NP_619597.1
• RefSeq Size: 1767 bp
• RefSeq ORF: 1206 bp
• Locus ID: 192156
• UniProt ID: Q99JF5
• Cytogenetics: 8 E1
• Gene Summary: Performs the first committed step in the biosynthesis of isoprenes.[UniProtKB/Swiss-Prot Function]