Rtn4ip1 (NM_130892) Mouse Tagged ORF Clone

CAT#: MG206202

Rtn4ip1 (tGFP-tagged) - Mouse reticulon 4 interacting protein 1 (Rtn4ip1)

Product Images

Circular map for MG206202

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rtn4ip1
• Synonyms: D10Ertd690e; NIMP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206202 representing NM_130892
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGTTTTGAAGACCTGTGTACTTAGAAGAAGTGCTTGTGCCGCCGCCTGCTTCTGGAGAAGGACAG
  TTATCCCAAAGCCACCATTTAGAGGCATTAGTACTACGTCTGCGAGGAGCACTGTCATGCCCGCTTGGGT
  GATAGATAAATATGGAAAGAATGAAGTCCTTCGATTCACCCAGAACATGATGTTACCTATCATACACTAT
  CCAAATGAAGTCATTATCAAAGTCCACGCTGCCAGTGTAAATCCTATAGATGTTAATATGAGAAGTGGTT
  ATGGAGCCACAGCCCTAAATATGAAGCGTGATCCCCTGCATATGAAAACCAAAGGAGAAGAGTTTCCTCT
  GACTCTGGGTCGGGATGTCTCTGGTGTGGTGATGGAGTGTGGGCTTGATGTGAAGTATTTCCAGCCTGGA
  GATGAGGTCTGGGCTGCAGTTCCTCCTTGGAAGCAAGGCACTCTCTCAGAATTTGTTGTGGTCAGTGGGA
  ATGAGGTTTCACACAAGCCCAAATCGCTTACTCATACTCAAGCTGCCTCTTTGCCATATGTGGCTCTCAC
  GGCCTGGTCTGCCATAAACAAGGTTGGCGGCCTGAGTGACAGGAACTGCAAGGGGAAGCGTGCCTTAATC
  TTGGGTGCGTCAGGTGGAGTTGGTACTTTTGCGATACAGGTAATGAAAGCATGGGGGGCTCATGTGACAG
  CCGTTTGCTCGAAAGATGCCAGTGAACTTGTGCGGAAGCTTGGAGCAGATGAGGTCATTGATTACACGTT
  GGGAAGTGTGGAAGAGCAATTGAAATCTCTAAAACTGTTTGACTTTATCCTTGATAACGTTGGTGGATCT
  ACTGAAACATGGGCTCTAAATTTTCTCAAGAAGTGGTCAGGAGCTACCTATGTGACTCTGGTGACTCCTT
  TCCTCCTGAACATGGACCGACTGGGTGTTGCTGATGGCATGTTGCAGACAGGAGTCACCGTGGGTACAAA
  GGCATTGAAGCATCTCTGGCAAGGAGTCCATTACAGATGGGCCTTCTTCATGGCCAGTGGTCCATATCTA
  GATGAAATTGCAGAACTGGTGGATGCAGGAAAGATCCGGCCAGTTATTGAGCGGACATTCCCTTTCTCTG
  AAGTCCCAGAAGCCTTCCTGAAGGTGGAGAGAGGCCACGCCCGAGGAAAGACTGTGGTTAATGTAGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206202 representing NM_130892
  Red=Cloning site Green=Tags(s)
  
  MGVLKTCVLRRSACAAACFWRRTVIPKPPFRGISTTSARSTVMPAWVIDKYGKNEVLRFTQNMMLPIIHY
  PNEVIIKVHAASVNPIDVNMRSGYGATALNMKRDPLHMKTKGEEFPLTLGRDVSGVVMECGLDVKYFQPG
  DEVWAAVPPWKQGTLSEFVVVSGNEVSHKPKSLTHTQAASLPYVALTAWSAINKVGGLSDRNCKGKRALI
  LGASGGVGTFAIQVMKAWGAHVTAVCSKDASELVRKLGADEVIDYTLGSVEEQLKSLKLFDFILDNVGGS
  TETWALNFLKKWSGATYVTLVTPFLLNMDRLGVADGMLQTGVTVGTKALKHLWQGVHYRWAFFMASGPYL
  DEIAELVDAGKIRPVIERTFPFSEVPEAFLKVERGHARGKTVVNVV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130892
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130892.5
• RefSeq Size: 2984 bp
• RefSeq ORF: 1191 bp
• Locus ID: 170728
• UniProt ID: Q924D0
• Cytogenetics: 10 23.12 cM
• Gene Summary: Plays a role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, and hence in the development of the inner retina and optic nerve (PubMed:26593267). Appears to be a potent inhibitor of regeneration following spinal cord injury (PubMed:12067236).[UniProtKB/Swiss-Prot Function]