Mapkapk2 (BC063064) Mouse Tagged ORF Clone

CAT#: MG206027

Mapkapk2 (tGFP-tagged) - Mouse MAP kinase-activated protein kinase 2 (cDNA clone MGC:67217 IMAGE:5687007)

Product Images

Circular map for MG206027

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mapkapk2
• Synonyms: AA960234; MAPKAP-K2; MK-2; MK2; Rps6kc1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206027 representing BC063064
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCGGGCTCTCCGGGCCAGACTCCGCCGGCGCCTTTCCCCAGCCCTCCACCGCCAGCCCCGGCCC
  AGCCGCCGCCGCCGTTCCCCCAGTTCCACGTCAAGTCGGGCCTGCAGATCCGAAAGAACGCCATCACCGA
  CGACTACAAGGTCACCAGCCAAGTGCTTGGCCTGGGCATCAACGGGAAGGTGCTGCGGATCTTCGACAAG
  AGAACCCAGCAAAAATTCGCCCTAAAGATGCTCCAGGACTGTCCGAAGGCGCGCAGAGAGGTGGAGCTGC
  ACTGGAGGGCCTCCCAGTGCCCACACATTGTGCACATCGTGGATGTCTATGAGAACCTGTATGCCGGGAG
  GAAGTGCCTGCTGATTGTCATGGAGTGTCTCGATGGTGGAGAGCTCTTTAGTCGAATCCAGGACCGAGGA
  GACCAGGCATTCACAGAAAGAGAGGCGTCAGAGATCATGAAGAGCATCGGCGAGGCCATCCAGTACCTGC
  ACTCGATCAACATTGCTCACCGGGATGTCAAGCCTGAGAACCTCTTATATACTTCCAAAAGGCCCAATGC
  CATTTTGAAACTCACTGATTTTGGCTTTGCCAAGGAAACCACCAGTCACAACTCTTTGACCACTCCGTGT
  TATACACCATACTATGTGGCTCCGGAAGTCCTGGGCCCGGAGAAGTATGACAAGTCCTGTGACATGTGGT
  CCTTGGGTGTCATCATGTATATTTTGCTGTGTGGGTATCCCCCCTTCTATTCCAATCACGGCCTTGCCAT
  CTCTCCGGGCATGAAGACTCGTATTCGAATGGGCCAGTATGAATTTCCTAACCCGGAGTGGTCAGAAGTA
  TCAGAAGAAGTGAAGATGCTTATCCGGAATCTGCTAAAAACAGAGCCCACCCAGAGAATGACCATCACAG
  AATTCATGAACCACCCCTGGATCATGCAATCTACGAAGGTCCCTCAGACTCCACTGCACACCAGCCGTGT
  CCTGAAGGAGGACAAGGAACGATGGGAGGATGTCAAGGAGGAGATGACCAGTGCCTTGGCCACGATGCGT
  GTTGACTATGAGCAGATCAAGATAAAGAAGATAGAAGACGCATCCAACCCTCTGCTTCTCAAGAGGCGGA
  AGAAAGCTCGTGCTGTGGAGGATGCGGCTCTCGCCCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206027 representing BC063064
  Red=Cloning site Green=Tags(s)
  
  MLSGSPGQTPPAPFPSPPPPAPAQPPPPFPQFHVKSGLQIRKNAITDDYKVTSQVLGLGINGKVLRIFDK
  RTQQKFALKMLQDCPKARREVELHWRASQCPHIVHIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRG
  DQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPC
  YTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEV
  SEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEMTSALATMR
  VDYEQIKIKKIEDASNPLLLKRRKKARAVEDAALAH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC063064
• ORF Size: 1160 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC063064, AAH63064
• RefSeq Size: 2586 bp
• RefSeq ORF: 1160 bp
• Locus ID: 17164
• Cytogenetics: 1 E4
• Gene Summary: Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities (By similarity). Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress following ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites (By similarity). Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.[UniProtKB/Swiss-Prot Function]