Slc14a1 (NM_028122) Mouse Tagged ORF Clone

CAT#: MG205999

Slc14a1 (tGFP-tagged) - Mouse solute carrier family 14 (urea transporter), member 1 (Slc14a1)

Product Images

Circular map for MG205999

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc14a1
• Synonyms: 2610507K20Rik; 3021401A05Rik; UT-B; Utb1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205999 representing NM_028122
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGATAGTCCCACCATGGTTAAAGTAGACCGGGGTGAAAACCAGATTTTATCATGCCGCGGGAGAA
  GGTGTGGCTTCAAAGTACTTGGCTACGTCACGGGTGACATGAAGGAATTCGCCAACTGGCTGAAAGACAA
  ACCCGTGGTGCTCCAGTTCATGGACTGGATACTTCGTGGCATATCCCAGGTGGTGTTTGTCAGCAACCCC
  ATCAGTGGAATCCTGATTCTGGTGGGACTTCTGGTCCAGAACCCCTGGTGGGCTCTCTGTGGCTGTGTAG
  GAACTGTGGTCTCCACTCTGACAGCCCTCTTGCTTAGCCAAGACAGATCGGCGATAGCAGCGGGGCTCCA
  AGGTTACAATGCCACCCTGGTAGGCATCCTCATGGCTGTCTTCTCAAACAAGGGCGACTATTTCTGGTGG
  CTGATATTCCCTGTATCTGCTATGTCTATGACTTGCCCGGTTTTCTCGAGCGCGTTGAGCTCCGTGCTCA
  GCAAGTGGGACCTGCCCGTCTTCACTCTCCCTTTCAACATGGCGTTGTCGATGTACCTGTCAGCCACAGG
  ACACTACAATACGTTTTTCCCAAGTAAACTCTTCACACCTGTCAGCTCCGTGCCCAACATCACGTGGTCT
  GAGCTCAGCGCCCTGGAGCTATTGAAGTCTCTTCCGGTGGGAGTCGGTCAGATATATGGCTGTGACAACC
  CGTGGACAGGCGGCATTTTCCTATGTGCTATCCTGCTCTCCTCCCCACTCATGTGCCTGCACGCTGCTAT
  TGGATCGTTGCTGGGTGTCATCGCGGGACTCAGTCTTGCAGCTCCATTTGAAGACATCTACTTTGGGCTC
  TGGGGTTTCAACAGCTCTCTGGCCTGCATTGCAATTGGAGGGATGTTCATGGCACTCACCTGGCAGACCC
  ACCTCCTGGCTCTTGCCTGTGCCCTGTTCACTGCCTACTTCGGAGCCTGTATGGCACACCTGATGGCTGT
  GGTTCACCTGCCAGCTTGTACCTGGTCCTTCTGTTTGGCCACACTACTCTTTCTCTTGCTGACCACGAAA
  AATCCCAACATCTACAGGATGCCCCTCAGCAAAGTTACCTACTCTGAGGAGAACCGCATCTTCTACCTCC
  AAAACAAGAAAAGGATGGTTGAAAGCCCCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205999 representing NM_028122
  Red=Cloning site Green=Tags(s)
  
  MEDSPTMVKVDRGENQILSCRGRRCGFKVLGYVTGDMKEFANWLKDKPVVLQFMDWILRGISQVVFVSNP
  ISGILILVGLLVQNPWWALCGCVGTVVSTLTALLLSQDRSAIAAGLQGYNATLVGILMAVFSNKGDYFWW
  LIFPVSAMSMTCPVFSSALSSVLSKWDLPVFTLPFNMALSMYLSATGHYNTFFPSKLFTPVSSVPNITWS
  ELSALELLKSLPVGVGQIYGCDNPWTGGIFLCAILLSSPLMCLHAAIGSLLGVIAGLSLAAPFEDIYFGL
  WGFNSSLACIAIGGMFMALTWQTHLLALACALFTAYFGACMAHLMAVVHLPACTWSFCLATLLFLLLTTK
  NPNIYRMPLSKVTYSEENRIFYLQNKKRMVESPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028122
• ORF Size: 3672 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028122.4, NP_082398.1
• RefSeq Size: 3580 bp
• RefSeq ORF: 1155 bp
• Locus ID: 108052
• UniProt ID: Q8VHL0
• Cytogenetics: 18 E3
• Gene Summary: Urea channel that facilitates transmembrane urea transport down a concentration gradient. A constriction of the transmembrane channel functions as selectivity filter through which urea is expected to pass in dehydrated form. The rate of urea conduction is increased by hypotonic stress. Plays an important role in the kidney medulla collecting ducts, where it allows rapid equilibration between the lumen of the collecting ducts and the interstitium, and thereby prevents water loss driven by the high concentration of urea in the urine. Facilitates urea transport across erythrocyte membranes. May also play a role in transmembrane water transport, possibly by indirect means.[UniProtKB/Swiss-Prot Function]