Il13ra2 (NM_008356) Mouse Tagged ORF Clone

CAT#: MG205982

Il13ra2 (tGFP-tagged) - Mouse interleukin 13 receptor, alpha 2 (Il13ra2)

Product Images

Circular map for MG205982

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Il13ra2
• Synonyms: CD213a; CD213a2; IL-13R-alpha-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205982 representing NM_008356
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTTGTGCATATCAGATGCTTGTGTTTCATTCTTCTTTGTACAATAACTGGCTATTCTTTGGAGA
  TAAAAGTTAATCCTCCTCAGGATTTTGAAATATTGGATCCTGGATTACTTGGTTATCTCTATTTGCAATG
  GAAACCTCCTGTGGTTATAGAAAAATTTAAGGGCTGTACACTAGAATATGAGTTAAAATACCGAAATGTT
  GATAGCGACAGCTGGAAGACTATAATTACTAGGAATCTAATTTACAAGGATGGGTTTGATCTTAATAAAG
  GCATTGAAGGAAAGATACGTACGCATTTGTCAGAGCATTGTACAAATGGATCAGAAGTACAAAGTCCATG
  GATAGAAGCTTCTTATGGGATATCAGATGAAGGAAGTTTGGAAACTAAAATTCAGGACATGAAGTGTATA
  TATTATAACTGGCAGTATTTGGTCTGCTCTTGGAAACCTGGCAAGACAGTATATTCTGATACCAACTATA
  CCATGTTTTTCTGGTATGAGGGCTTGGATCATGCCTTACAGTGTGCTGATTACCTCCAGCATGATGAAAA
  AAATGTTGGATGCAAACTGTCCAACTTGGACTCATCAGACTATAAAGATTTTTTTATCTGTGTTAATGGA
  TCTTCAAAGTTGGAACCCATCAGATCCAGCTATACAGTTTTTCAACTTCAAAATATAGTTAAACCATTGC
  CACCAGAATTCCTTCATATTAGTGTGGAGAATTCCATTGATATTAGAATGAAATGGAGCACACCTGGAGG
  ACCCATTCCACCAAGGTGTTACACTTATGAAATTGTGATCCGAGAAGACGATATTTCCTGGGAGTCTGCC
  ACAGACAAAAACGATATGAAGTTGAAGAGGAGAGCAAATGAAAGTGAAGACCTATGCTTTTTTGTAAGAT
  GTAAGGTCAATATATATTGTGCAGATGATGGAATTTGGAGCGAATGGAGTGAAGAGGAATGTTGGGAAGG
  TTACACAGGGCCAGACTCAAAGATTATTTTCATAGTACCAGTTTGTCTTTTCTTTATATTCCTTTTGTTA
  CTTCTTTGCCTTATTGTGGAGAAGGAAGAACCTGAACCCACATTGAGCCTCCATGTGGATCTGAACAAAG
  AAGTGTGTGCTTATGAAGATACCCTCTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205982 representing NM_008356
  Red=Cloning site Green=Tags(s)
  
  MAFVHIRCLCFILLCTITGYSLEIKVNPPQDFEILDPGLLGYLYLQWKPPVVIEKFKGCTLEYELKYRNV
  DSDSWKTIITRNLIYKDGFDLNKGIEGKIRTHLSEHCTNGSEVQSPWIEASYGISDEGSLETKIQDMKCI
  YYNWQYLVCSWKPGKTVYSDTNYTMFFWYEGLDHALQCADYLQHDEKNVGCKLSNLDSSDYKDFFICVNG
  SSKLEPIRSSYTVFQLQNIVKPLPPEFLHISVENSIDIRMKWSTPGGPIPPRCYTYEIVIREDDISWESA
  TDKNDMKLKRRANESEDLCFFVRCKVNIYCADDGIWSEWSEEECWEGYTGPDSKIIFIVPVCLFFIFLLL
  LLCLIVEKEEPEPTLSLHVDLNKEVCAYEDTLC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008356
• ORF Size: 1149 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008356.4
• RefSeq Size: 1567 bp
• RefSeq ORF: 1152 bp
• Locus ID: 16165
• UniProt ID: O88786
• Cytogenetics: X 68.46 cM
• Gene Summary: This gene encodes a receptor protein that binds to interleukin 13 (IL-13) with very high affinity. The encoded protein acts as a decoy receptor, and does not elicit any signal upon the binding of IL-13. Mice lacking the encoded protein exhibit increased levels of serum immunoglobulins, immune-dependent production of interferon gamma and, increased bone marrow macrophage progenitor frequency. Macrophages lacking the encoded protein release less nitric oxide and IL-12 in response to lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]