Elmod3 (NM_144917) Mouse Tagged ORF Clone

CAT#: MG205956

Elmod3 (tGFP-tagged) - Mouse RNA binding motif and ELMO domain 1 (Rbed1)

Product Images

Circular map for MG205956

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Elmod3
• Synonyms: AI844780; C330008I15Rik; Rbed1; RBM29
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205956 representing NM_144917
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAAACATCTTGCTCTTTTTTTATTGAGAAAGAATTTCAAGATGGGCAGCTAGAAAATGTCTCTG
  CTGGGCTCTCTTCATCGTACAAGGACAAGGGTGCACTCATGGCTTTCAGAGGAATTCCTATCTCCGAGCT
  GACGAACCATGGCATTCTCCAGGCCCTGACGGCAGAAACCAATGGGTGGCAGCCAGGTGTTGTGAGTGAA
  GAGGTCCTCAGAGCCCAGGAAGAGTGGGAAGTGGTGGACACCATCCATCCAGACATAGAGAGTGGAGTCC
  ACTGCCAGCAGCCTGGCCAGCTCATCTCCTTCAACGAGGCCCTGGAGCACTTCCAGAGTGTGGACCTTTC
  CTCTTTCAAGAAAAGAATCCAGCCCACTATTCAAAGGACTGGGCTCGCCGCCCTCCGGCATTGCCTTTTC
  GGGCCGCCAAAGCTGCACCAGGGTCTTCGGGAAGAAAGAGACTTGGTTCTCACCATTGCTCAGTGTGGCC
  TGGACAGCCAAAACCCAACGCATGGCCGAGTGCTCCAGACCATCTACAAGAAGCTGACTGGCTCCAAGTT
  CGACTGTGCCCTTCACGGAGACCATTGGGAAGACCTGGGCTTCCAGGGAGCAAACCCAGCCACAGACCTC
  CGGGGGGCAGGCTTCCTGGCCCTCCTGCACCTACTCTACCTGGTCATGGACTCAAAGACGTTTCTGATGG
  CCCAGGAGATCTTCCGCCTGTCTCATCACCATATCCAGCAATTCCCCTTCTGTTTGATGTCTGTGAACAT
  CACCCGCATTGCCATCCAGGCCTTGAGAGAGGAGTGTCTCTCCAGGGAGTGTAATCGGCGGCAGAAGGTC
  ATCCCGGTGGTGAACAGCTTCTACGCGGCTACTTTCCTTCATCTGGCTCGCGTGTGGAGGACCCAGCAGA
  AGACTATTTTAGACTCAGGCTTTGTCCTCAAAGACTTGGAAGCGTTGGCCAAGAAGAGCCCAAAACGACT
  GCTCAAGACCCTGGATGACTACCTCGCCCAGGTGTCAAAGGGACAGACCTCCTTGTTGGAGGCACACAAG
  CGCCCTGGATCACAGGGACCTCACTCCAGGGATCTCACTTTCACAGGCGTGTGTGACCTGCAGTCTCACT
  CATTCGAGAGTGCAGGGTTGATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205956 representing NM_144917
  Red=Cloning site Green=Tags(s)
  
  MSETSCSFFIEKEFQDGQLENVSAGLSSSYKDKGALMAFRGIPISELTNHGILQALTAETNGWQPGVVSE
  EVLRAQEEWEVVDTIHPDIESGVHCQQPGQLISFNEALEHFQSVDLSSFKKRIQPTIQRTGLAALRHCLF
  GPPKLHQGLREERDLVLTIAQCGLDSQNPTHGRVLQTIYKKLTGSKFDCALHGDHWEDLGFQGANPATDL
  RGAGFLALLHLLYLVMDSKTFLMAQEIFRLSHHHIQQFPFCLMSVNITRIAIQALREECLSRECNRRQKV
  IPVVNSFYAATFLHLARVWRTQQKTILDSGFVLKDLEALAKKSPKRLLKTLDDYLAQVSKGQTSLLEAHK
  RPGSQGPHSRDLTFTGVCDLQSHSFESAGLI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144917
• ORF Size: 1143 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144917.5
• RefSeq Size: 2160 bp
• RefSeq ORF: 1146 bp
• Locus ID: 232089
• UniProt ID: Q91YP6
• Cytogenetics: 6 C1
• Gene Summary: Acts as a GTPase-activating protein (GAP) for ARL2 with low specific activity.[UniProtKB/Swiss-Prot Function]