Rom1 (NM_009073) Mouse Tagged ORF Clone

CAT#: MG205332

Rom1 (tGFP-tagged) - Mouse rod outer segment membrane protein 1 (Rom1)

Product Images

Circular map for MG205332

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rom1
• Synonyms: M101156; Rgsc1156; Rom-1; Rosp1; Tspan23
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205332 representing NM_009073
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGGTGCTGCCCGTGGTGCTGCCCCTCCAACCCCGTATCCGTTTGGCACAGGGCATCTGGCTCC
  TCTCCTGGCTGCTGGCATTGGTCGGTGGCCTCACCCTCCTTTGTAGCGGGCACCTTCTGGTACAGCTGGG
  GCACCTTGGCACCTTCCTGGCACCCTCTTGTTCATTCCCTGCTCTGCCCCAGACTGCCCTGGCAGCGGGA
  ACGGTGGCTCTAGGCACAGGGCTAGGAGGCGCAGGAGCCAGCCGGGCAAGTCTGGATGCAGCTCAATACC
  CCCCCTGGAGAGGGGTCTTGACTCCACTGCTAGCGGTTGGCACAGCTGCAGGTGGGGGGCTGCTGACCCT
  TGCCCTGGGGCTAGCCCTGGCTTTGCCAGTAAGTCTCAACCAGGGACTGGAGGAGGGCCTGGAGGCTGCC
  TTGGCTCACTACAAGGACACAGAGGTGCCTGGACGCTGTCAGGCCAAACGTCTGATGGATGAGTTGCAGT
  TGAGGTACCATTGCTGCGGGCGCCATGGCTACAAGGATTGGTTTGGTGTTCAGTGGGTCAGCAACCGTTA
  CTTGGACCCCAGTGACCAAGATGTAGTTGACCGGATCCAGAGCAATGTGGAAGGCTTATATCTGATTGAT
  GGCGTCCCCTTCTCCTGTTGTAATCCCCACTCACCCCGGCCTTGCCTGCAAAGCCAACTCTCGGACCCCT
  ATGCCCATCCACTCTTCGATCCTCGGCAGCCCAACCTAAACCTCTGGGCCCAAGGGTGCCATGAAGTGCT
  GCTGGAACACCTGCAGGGTTTATCAGGCACACTGGGAAGTATTCTGGCTGTCACCTTATTGCTGCAGATT
  CTAGTGCTCCTTGGTTTGCGGTATTTGCAGACAGCGCTGGAGGGCCTTGGAGGAGTCATTGATGGGGAAG
  GAGAGGCCCAGGGCTATCTTTTTCCTGGTGGGCTGAAAGACATACTGAAAACTGCATGGCTACAGGGAGG
  GCTTGCCCACAAGCCAGCACCTGAGGAGGCCCCACCAGATGAAGAACCTCCCAAGGAAGTTCTAGCTGAG
  GCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205332 representing NM_009073
  Red=Cloning site Green=Tags(s)
  
  MAPVLPVVLPLQPRIRLAQGIWLLSWLLALVGGLTLLCSGHLLVQLGHLGTFLAPSCSFPALPQTALAAG
  TVALGTGLGGAGASRASLDAAQYPPWRGVLTPLLAVGTAAGGGLLTLALGLALALPVSLNQGLEEGLEAA
  LAHYKDTEVPGRCQAKRLMDELQLRYHCCGRHGYKDWFGVQWVSNRYLDPSDQDVVDRIQSNVEGLYLID
  GVPFSCCNPHSPRPCLQSQLSDPYAHPLFDPRQPNLNLWAQGCHEVLLEHLQGLSGTLGSILAVTLLLQI
  LVLLGLRYLQTALEGLGGVIDGEGEAQGYLFPGGLKDILKTAWLQGGLAHKPAPEEAPPDEEPPKEVLAE
  A
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009073
• ORF Size: 1053 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009073.4
• RefSeq Size: 1511 bp
• RefSeq ORF: 1056 bp
• Locus ID: 19881
• UniProt ID: P32958
• Cytogenetics: 19 6.03 cM
• Gene Summary: May function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. It is essential for disk morphogenesis.[UniProtKB/Swiss-Prot Function]