Lpar2 (NM_020028) Mouse Tagged ORF Clone

CAT#: MG205261

Lpar2 (tGFP-tagged) - Mouse endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor 4 (Edg4)

Product Images

Circular map for MG205261

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lpar2
• Synonyms: Edg4; IPA2; LPA2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205261 representing NM_020028
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCAGTGCTACTACAACGAGACCATCGGCTTTTTCTATAATAACAGCGGCAAGGAGCTCAGCCTTC
  ACTGGCGCCCCAAAGATGTGGTGGTGGTGGCCCTGGGGCTGACAGTCAGTGTGCTGGTATTGCTGACCAA
  TCTGCTGGTTATTGCAGCCATCGCCTCCAACCGACGCTTCCACCAGCCCATATACTACCTGCTTGGCAAC
  TTGGCTGCGGCTGACCTCTTCGCTGGCATGGCCTACCTCTTCCTCATGTTCCATACTGGCCCACGCACTG
  CCAGGCTCTCCATCAAAGGCTGGTTCCTGCGACAGGGCCTGCTGGACACCAGCCTCACGGCGTCAGTGGC
  CACACTGCTGGCCATCGCTGTGGAACGGCACCGCAGTGTGATGGCGGTACAGCTACACAGCCGCCTGCCC
  CGGGGCCGTGTGGTCACACTCATCGTGGGTGTGTGGGCGGCTGCACTGGGTCTGGGGTTGCTACCTGCAC
  ACTTCTGGCACTGCCTCTGTGACTTGGACAGTTGCTCACGCATGGTGCCCCTGTTCAGCCGCTCCTACTT
  GGCTGCGTGGGCCCTATCCAGCCTGCTTGTCTTCCTACTCATGGTAGCTGTCTACACACGAATTTTCTTC
  TATGTGCGTAGACGGGTGGAACGCATGGCGGAGCACGTCAGCTGCCATCCCCGCTACCGAGAGACCACAC
  TCAGCCTAGTCAAGACGGTTGTCATCATTCTGGGGGCATTTGTGGTGTGCTGGACACCGGGCCAGGTGGT
  GCTGCTCCTGGATGGCCTGGACTGTAAGTCCTGCAACGTTCTGGCTGTGGAGAAGTACTTCCTGCTCCTG
  GCTGAGGCCAACTCACTGGTCAATGCAGTGGTATATTCCTGCCGAGATGCTGAGATGCGCCGCACCTTCC
  GCCGCCTTCTCTGCTGCATGTGTCTCCGCTGGTCCAGCCACAAGTCTGCCCGTTACTCAGCTTCTGCCCA
  GACGGGTGCCAGCACGCGGATCATGCTTCCTGAGAATGGCCGCCCACTGATGGACTCCACCCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205261 representing NM_020028
  Red=Cloning site Green=Tags(s)
  
  MGQCYYNETIGFFYNNSGKELSLHWRPKDVVVVALGLTVSVLVLLTNLLVIAAIASNRRFHQPIYYLLGN
  LAAADLFAGMAYLFLMFHTGPRTARLSIKGWFLRQGLLDTSLTASVATLLAIAVERHRSVMAVQLHSRLP
  RGRVVTLIVGVWAAALGLGLLPAHFWHCLCDLDSCSRMVPLFSRSYLAAWALSSLLVFLLMVAVYTRIFF
  YVRRRVERMAEHVSCHPRYRETTLSLVKTVVIILGAFVVCWTPGQVVLLLDGLDCKSCNVLAVEKYFLLL
  AEANSLVNAVVYSCRDAEMRRTFRRLLCCMCLRWSSHKSARYSASAQTGASTRIMLPENGRPLMDSTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020028
• ORF Size: 1044 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020028.3, NP_064412.2
• RefSeq Size: 5701 bp
• RefSeq ORF: 1047 bp
• Locus ID: 53978
• Cytogenetics: 8 33.91 cM