Cdk20 (NM_053180) Mouse Tagged ORF Clone

CAT#: MG205221

Cdk20 (tGFP-tagged) - Mouse cell cycle related kinase (Ccrk)

Product Images

Circular map for MG205221

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cdk20
• Synonyms: 4932702G04Rik; AU019450; AW558027; Ccrk; CDCH; p42; PNQLARE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205221 representing NM_053180
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAGTATTGCATCCTCGGTCGCATCGGGGAGGGCGCCCACGGCATCGTCTTCAAAGCCAAGCACG
  TAGAGACTGGCGAGATCGTTGCCCTCAAGAAGGTGGCCTTGCGGCGGCTGGAGGATGGTATTCCTAACCA
  GGCCCTCAGAGAAATCAAGGCTCTGCAGGAGATAGAGGACAGTCAATATGTGGTGCAGCTGAAGGCTGTG
  TTCCCACATGGTGCGGGCTTTGTGCTGGCTTTCGAATTTATGCTGTCGGACCTGGCAGAGGTGGTGCGCC
  ATGCCCAGAGGCCCCTGGCCCCAGCACAAGTCAAGAGCTACCTGCAGATGCTGCTCAAAGGTGTTGCGTT
  TTGCCATGCCAACAACATTGTGCATCGGGACCTGAAGCCTGCTAACCTGCTCATCAGTGCCTCAGGCCAG
  CTCAAGATAGCTGACTTTGGCCTGGCCCGGGTCTTCTCTCCGGATGGTGGTCGCCTCTACACACATCAGG
  TGGCCACCAGGTGGTACCGAGCTCCTGAACTCCTGTATGGCGCTCGGCAGTATGACCAGGGCGTTGACCT
  ATGGGCTGTGGGCTGCATCATGGGAGAGCTGTTGAATGGGTCCCCCCTGTTCCCGGGCGAAAACGACATT
  GAACAACTGTGCTGTGTGCTTCGCATCCTGGGTACCCCGAGTCCTCGAGTCTGGCCGGAGATCACAGAGC
  TGCCTGACTACAACAAGATCTCCTTCAAGGAGCAGGCACCAGTGCCCCTGGAGGAGGTGCTGCCTGATGC
  CTCTCCCCAGGCCTTGGACCTGCTGGGCCAGTTCCTCCTCTACCCTCCACGACAGCGTATTGCAGCCTCC
  CAGGCCCTTCTGCATCAGTACTTCTTCACAGCGCCTCTGCCTGCCCATCCATCCGAGCTGCCAATTCCTC
  AGCGCCCAGGGGGACCTGCACCCAAGGCTCACCCAGGGCCCCCCCATGTCCACGACTTCCATGTGGATCG
  ACCTCTTGAGGAGTCACTGTTGAACCCAGAACTGATTCGGCCCTTCATCCCAGAGGGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205221 representing NM_053180
  Red=Cloning site Green=Tags(s)
  
  MDQYCILGRIGEGAHGIVFKAKHVETGEIVALKKVALRRLEDGIPNQALREIKALQEIEDSQYVVQLKAV
  FPHGAGFVLAFEFMLSDLAEVVRHAQRPLAPAQVKSYLQMLLKGVAFCHANNIVHRDLKPANLLISASGQ
  LKIADFGLARVFSPDGGRLYTHQVATRWYRAPELLYGARQYDQGVDLWAVGCIMGELLNGSPLFPGENDI
  EQLCCVLRILGTPSPRVWPEITELPDYNKISFKEQAPVPLEEVLPDASPQALDLLGQFLLYPPRQRIAAS
  QALLHQYFFTAPLPAHPSELPIPQRPGGPAPKAHPGPPHVHDFHVDRPLEESLLNPELIRPFIPEG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053180
• ORF Size: 1038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053180.2, NP_444410.1
• RefSeq Size: 1854 bp
• RefSeq ORF: 1041 bp
• Locus ID: 105278
• UniProt ID: Q9JHU3
• Cytogenetics: 13 B3
• Gene Summary: Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160' (By similarity). Required for high-level Shh responses in the developing neural tube. Together with TBC1D32, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling.[UniProtKB/Swiss-Prot Function]