Hyal2 (BC060047) Mouse Tagged ORF Clone

CAT#: MG204921

Hyal2 (tGFP-tagged) - Mouse hyaluronoglucosaminidase 2 (cDNA clone MGC:64716 IMAGE:5685601)

Product Images

Circular map for MG204921

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hyal2
• Synonyms: AI256841; AU020858; AW555733
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204921 representing BC060047
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGCAGGACTAGGTCCCATCATCACACTGGCCCTAGTGCTGGAGGTAGCATGGGCCGGGGAGCTTA
  AGCCCACAGCGCCGCCCATCTTCACTGGCCGACCCTTTGTGGTAGCATGGAACGTGCCCACACAAGAATG
  TGCCCCACGCCACAAAGTGCCACTGGACCTTAGGGCCTTCGATGTGAAGGCTACACCGAATGAGGGTTTT
  TTCAACCAGAATATCACCACCTTCTACTACGACCGTCTAGGCCTGTATCCACGTTTTGATGCAGCCGGGA
  CATCTGTGCATGGCGGTGTGCCTCAGAACGGCAGCCTCTGTGCACACCTGCCCATGCTGAAGGAATCTGT
  GGAACGCTACATCCAGACCCAGGAGCCTGGGGGGCTGGCAGTCATTGACTGGGAGGAATGGCGGCCTGTA
  TGGGTTCGAAACTGGCAGGAGAAAGATGTTTACCGACAGTCTTCACGCCAGCTGGTGGCCAGTCGGCACC
  CTGACTGGCCATCAGACAGAGTAATGAAGCAGGCCCAGTACGAGTTTGAGTTCGCCGCTCGGCAGTTCAT
  GTTGAACACTCTCCGTTACGTCAAGGCAGTCAGACCCCAGCACCTGTGGGGCTTCTACCTCTTTCCTGAC
  TGCTACAATCACGATTATGTACAGAACTGGGAGAGCTACACGGGCCGCTGTCCCGATGTGGAGGTGGCAC
  GGAACGACCAGCTGGCCTGGCTCTGGGCTGAGAGCACGGCTCTCTTTCCCTCTGTGTACCTGGACGAGAC
  ACTGGCGTCCTCCGTACACAGCCGCAACTTTGTCAGTTTCCGTGTTCGGGAGGCCCTTCGAGTGGCTCAC
  ACCCACCATGCCAACCACGCCCTCCCCGTGTACGTCTTCACGCGTCCCACATACACCCGAGGACTCACGG
  GACTGAGCCAGGTACGTGTTCTCCCTTCCTCCGGGAGCCACTGCATGCTTGTGTTATCGGGGACCAGAAG
  TACTGTTCCATATCTGTTT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204921 representing BC060047
  Red=Cloning site Green=Tags(s)
  
  MRAGLGPIITLALVLEVAWAGELKPTAPPIFTGRPFVVAWNVPTQECAPRHKVPLDLRAFDVKATPNEGF
  FNQNITTFYYDRLGLYPRFDAAGTSVHGGVPQNGSLCAHLPMLKESVERYIQTQEPGGLAVIDWEEWRPV
  WVRNWQEKDVYRQSSRQLVASRHPDWPSDRVMKQAQYEFEFAARQFMLNTLRYVKAVRPQHLWGFYLFPD
  CYNHDYVQNWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSVHSRNFVSFRVREALRVAH
  THHANHALPVYVFTRPTYTRGLTGLSQVRVLPSSGSHCMLVLSGTRSTVPYLF
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC060047
• ORF Size: 1001 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC060047
• RefSeq Size: 2996 bp
• RefSeq ORF: 1001 bp
• Locus ID: 15587
• Cytogenetics: 9 58.12 cM
• Gene Summary: Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R (By similarity).[UniProtKB/Swiss-Prot Function]