Eif2s2 (NM_026030) Mouse Tagged ORF Clone

CAT#: MG204882

Eif2s2 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2)

Product Images

Circular map for MG204882

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Eif2s2
• Synonyms: 38kDa; 2810026E11Rik; AA408636; AA571381; AA986487; AW822225; D2Ertd303e; EIF2; EIF2B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204882 representing NM_026030
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGGGACGAGATGATTTTTGATCCTACTATGAGCAAAAAGAAAAAGAAGAAGAAGAAGCCTTTTA
  TGTTAGATGAAGAAGGTGATGCCCAGACAGAAGAAACCCAGCCCTCAGAGACAAAAGAAGTGGAGCCAGA
  ACCAACTGAAGAAAAAGACGTGGACGCTGATGAGGAAGACAGTAGGAAGAAAGATGCTTCTGATGACTTA
  GATGATTTGAACTTCTTTAATCAAAAGAAAAAGAAGAAAAAGACAAAAAAGATATTTGACATTGATGAAG
  CTGAAGAAGCTATAAAGGATGTTAAGATTGAGAGTGATGCTCAAGAGCCAGCCGAGCCAGAGGATGACCT
  TGACATTATGCTTGGCAACAAAAAAAAGAAAAAGAAGAATGTTAAATTCCCAGAAGAAGATGAAATACTA
  GAAAAAGACGAAGCTTTAGAAGATGAAGACAGCAAAAAAGATGATGGCATTTCATTCAGTAGCCAAACTG
  CTTGGGCAGGCTCAGAAAGAGACTACACATATGAGGAGTTGCTGAACCGAGTGTTCAACATCATGAGAGA
  AAAGAATCCAGATATGGTTGCTGGAGAGAAGAGGAAATTTGTTATGAAACCTCCACAGGTCGTCCGAGTA
  GGAACCAAGAAAACTTCTTTTGTCAATTTTACAGATATCTGTAAACTATTACATCGTCAACCCAAACATC
  TCCTTGCATTTTTATTGGCAGAATTGGGTACAAGTGGTTCTATAGATGGTAATAACCAACTTGTAATCAA
  AGGAAGATTCCAACAGAAACAAATAGAAAATGTCTTGAGAAGATATATCAAGGAATATGTCACTTGTCAC
  ACATGCCGGTCACCGGACACAATCCTACAGAAGGACACCCGACTCTATTTCTTACAATGTGAAACTTGTC
  ATTCTCGATGCTCTGTTGCCAGTATCAAAACTGGCTTCCAGGCTGTCACAGGCAAGCGAGCACAGCTCCG
  TGCCAAAGCTAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204882 representing NM_026030
  Red=Cloning site Green=Tags(s)
  
  MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDAQTEETQPSETKEVEPEPTEEKDVDADEEDSRKKDASDDL
  DDLNFFNQKKKKKKTKKIFDIDEAEEAIKDVKIESDAQEPAEPEDDLDIMLGNKKKKKKNVKFPEEDEIL
  EKDEALEDEDSKKDDGISFSSQTAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRV
  GTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCH
  TCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026030
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026030.2, NP_080306.1
• RefSeq Size: 2513 bp
• RefSeq ORF: 996 bp
• Locus ID: 67204
• UniProt ID: Q99L45
• Cytogenetics: 2 76.89 cM
• Gene Summary: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).[UniProtKB/Swiss-Prot Function]