Mpped1 (NM_172610) Mouse Tagged ORF Clone

CAT#: MG204776

Mpped1 (tGFP-tagged) - Mouse metallophosphoesterase domain containing 1 (Mpped1)

Product Images

Circular map for MG204776

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mpped1
• Synonyms: 6230403M19; AW456965; D15Bwg0669e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204776 representing NM_172610
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCGCCCTAGGTGGGATCCTGGCATCCTGAAGGCTGAAGCCCTGGCCCTGCTCCCCTGTGGCCTGG
  GCATGGCGTTCTCTCAGTCTCACGTGATGGCCTCTCGACGGCACCAACATGGCCGACTCATCATCGAGGT
  GGACGAATACAGCTCCAATCCCACCCAGGCCTTCACCTTCTACAATATCAACCAGGGCCGCTTCCAGCCA
  CCTCACGTGCAGATGGTGGACCCAGTGCCTCACGATGCCCCAAAGCCACCTGGCTACACACGTTTTGTCT
  GTGTTTCTGACACTCACTCAAGGACAGATCCCATTCAAATGCCCTACGGCGATGTGCTGATCCACGCTGG
  GGACTTCACGGAACTGGGGCTCCCAAGCGAGGTGAAGAAGTTCAATGAATGGCTGGGCAGCCTGCCCTAT
  GAGTACAAGATCGTGATTGCGGGCAACCACGAGCTGACCTTCGACCAGGAGTTCATGGCCGACCTCATCA
  AGCAGGACTTTTATTATTTCCCGTCCGTGTCCAAGCTGAAGCCGGAGAACTATGAGAACGTGCAGTCTCT
  GCTGACCAACTGCATCTACCTGCAGGACTCAGAGGTCACGGTGCGTGGCTTCCGGATCTACGGCTCCCCG
  TGGCAGCCCTGGTTCTATGGCTGGGGATTCAACCTCCCGAGAGGCCAAGCGCTCCTGGAGAAGTGGAATC
  TCATCCCAGAAGGAGTAGACATCCTTATAACTCATGGACCACCACTGGGCTTTCTAGACTGGGTCCCCAA
  GAAGATGCAGCGTGTGGGCTGTGTGGAGCTGCTCAACACAGTCCAGAGGCGGGTCCAGCCACGGCTGCAC
  GTCTTTGGCCACATCCATGAAGGGTATGGCGTCATGGCAGACGGGACCACCACCTATGTGAACGCGTCGG
  TGTGCACTGTGAACTATCAGCCTGTGAATCCCCCCATAGTCATCGACCTGCCCACACCCCGGAACTCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204776 representing NM_172610
  Red=Cloning site Green=Tags(s)
  
  MWRPRWDPGILKAEALALLPCGLGMAFSQSHVMASRRHQHGRLIIEVDEYSSNPTQAFTFYNINQGRFQP
  PHVQMVDPVPHDAPKPPGYTRFVCVSDTHSRTDPIQMPYGDVLIHAGDFTELGLPSEVKKFNEWLGSLPY
  EYKIVIAGNHELTFDQEFMADLIKQDFYYFPSVSKLKPENYENVQSLLTNCIYLQDSEVTVRGFRIYGSP
  WQPWFYGWGFNLPRGQALLEKWNLIPEGVDILITHGPPLGFLDWVPKKMQRVGCVELLNTVQRRVQPRLH
  VFGHIHEGYGVMADGTTTYVNASVCTVNYQPVNPPIVIDLPTPRNS
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172610
• ORF Size: 978 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172610.3
• RefSeq Size: 3256 bp
• RefSeq ORF: 981 bp
• Locus ID: 223726
• UniProt ID: Q91ZG2
• Cytogenetics: 15 39.44 cM
• Gene Summary: May have metallophosphoesterase activity (in vitro).[UniProtKB/Swiss-Prot Function]