Xrcc4 (NM_028012) Mouse Tagged ORF Clone

CAT#: MG204755

Xrcc4 (tGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4)

Product Images

Circular map for MG204755

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Xrcc4
• Synonyms: 2310057B22Rik; AW413319; AW545101
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204755 representing NM_028012
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGGAAAGTAAGCAGAATCTACCTTGCTTCTGAACCCAACGTACCTTATTTTCTGCAAGTGTCTT
  GGGAGAGAGCAATAGGATCCGGCTTTGTTATTACACTTACTGACGGCCATTCAGCCTGGACTGCAACAGT
  TTCTGAATTGGAGATTTCCCAAGAAGCTGATGACATGGCTATGGAGAAAGGAAAATACATTGATGAGCTG
  AGAAAGGCACTGGTGCCAGGGTCAGGAGCAGCTGGTACATACAAGTTCCTCTTTTCTAAGGAGTCTCAGC
  ATTTCTCCCTTGAGAAAGAACTGAAAGATGTTTCATTCAGACTTGGTTCCTTCAACCTAGACAAAGTTTC
  AAACTCAGCTGAAGTCATAAGAGAACTCATTTGTTACTGCCTGGACACCATTACAGAAAAGCAAGCCAAA
  AACGAGCACCTGCAGAAAGAAAATGAAAGGCTCCTGAGAGACTGGAATGATGTTCAAGGCCGATTTGAGA
  AATGTGTGAGTGCCAAAGAAGCCTTGGAGGCTGATTTGTATCAAAGATTTATCTTGGTGCTGAATGAGAA
  GAAAACAAAGATCCGGAGCTTGCATAAATTGCTAAATGAAGTCCAGCAGCTGGAGGAGAGTACCAAACCT
  GAAAGGGAAAATCCGTGTTCTGACAAAACACCTGAGGAGCATGGGCTTTATGATGGAAGCACCGATGAAG
  AAAGTGGAGCCCCTGTGCAGGCTGCAGAAACTCTTCATAAGGATGATTCCATATTTTCAAGTCCGGATGT
  CACTGACATTGCACCAAGTAGGAAGAGGAGACACCGAATGCAGAAGAATCTTGGGACAGAACCTAAAATG
  GCTCCACAGGAGTTGCCGCTACAGGAGAAGGAAAGGCTTGCCTCTTCACTACCCCAGACCTTGAAAGAGG
  AGAGCACCTCTGCTGAAAACATGTCTTTAGAAACCCTGAGAAACAGCAGCCCAGAAGATCTCTTTGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204755 representing NM_028012
  Red=Cloning site Green=Tags(s)
  
  MERKVSRIYLASEPNVPYFLQVSWERAIGSGFVITLTDGHSAWTATVSELEISQEADDMAMEKGKYIDEL
  RKALVPGSGAAGTYKFLFSKESQHFSLEKELKDVSFRLGSFNLDKVSNSAEVIRELICYCLDTITEKQAK
  NEHLQKENERLLRDWNDVQGRFEKCVSAKEALEADLYQRFILVLNEKKTKIRSLHKLLNEVQQLEESTKP
  ERENPCSDKTPEEHGLYDGSTDEESGAPVQAAETLHKDDSIFSSPDVTDIAPSRKRRHRMQKNLGTEPKM
  APQELPLQEKERLASSLPQTLKEESTSAENMSLETLRNSSPEDLFD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028012
• ORF Size: 978 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028012.1, NP_082288.1
• RefSeq Size: 1457 bp
• RefSeq ORF: 981 bp
• Locus ID: 108138
• UniProt ID: Q924T3
• Cytogenetics: 13 C3
• Gene Summary: Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends (By similarity).[UniProtKB/Swiss-Prot Function]