Sfxn1 (NM_027324) Mouse Tagged ORF Clone

CAT#: MG204649

Sfxn1 (tGFP-tagged) - Mouse sideroflexin 1 (Sfxn1)

Product Images

Circular map for MG204649

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sfxn1
• Synonyms: 2810002O05Rik; A930015P12Rik; f
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204649 representing NM_027324
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGGGAAGTGCCACCCAACATTAACATCAAGGAGCCTCGATGGGACCAGAGCACGTTCATTGGCC
  GAGCCAGTCACTTCTTCACGGTTACTGATCCCAGAAATATCCTTTTAACGAACGAACAGCTAGAGAATGC
  GAGGAAAGTGGTGCATGACTACAGGCAAGGAATCGTTCCTGCCGGCCTCACGGAAAATGAGCTATGGAGA
  GCGAAGTACGCGTACGACTCGGCCTTCCATCCTGACACCGGTGAAAAGATGACTCTGATAGGAAGAATGT
  CAGCTCAGGTCCCGATGAACATGACCATCACAGGCTGCATGATGACCTTCTACCGGACCACACCGGCTGT
  GCTTTTCTGGCAGTGGATAAACCAGTCCTTCAATGCCGTGGTCAACTACACCAACAGAAGCGGTGACGCT
  CCCCTCACTGTGAATGAGCTGGGAACGGCTTATGTTTCTGCAACCACTGGCGCTGTGGCAACGGCCCTAG
  GACTCAATGCCTTAACCAAGCGTGTCTCCCCACTGATAGGACGTTTTGTTCCCTTCGCTGCTGTAGCTGC
  TGCTAACTGCATTAACATCCCACTGATGAGACAAAGGGAGCTGAAGGTTGGTATTCCTGTCACTGATGAA
  AATGGCACTCGCTTGGGAGAATCGACCAACGCAGCAAAGCAAGCCATCACGCAGGTGGTCATCTCCAGGA
  TCCTCATGGCAGCCCCGGGCATGGCCATCCCTCCGTTTATCATGAACACCTTGGAAAAGAAAGCCTTTTT
  AAAGAGGTTCCCGTGGATGAGTGCGCCAATCCAAGTCACGTTGGTTGGCTTTTGTTTGGTATTCGCCACC
  CCCCTGTGCTGTGCTCTGTTTCCTCAGAAGAGTTCCATGTCTGTGACAAGCTTGGAGGATGAGTTGCAAG
  CCAGCATCCAAAGGACTCATCCTGAAATACGGCGTGTGTACTTTAACAAGGGCCTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204649 representing NM_027324
  Red=Cloning site Green=Tags(s)
  
  MSGEVPPNINIKEPRWDQSTFIGRASHFFTVTDPRNILLTNEQLENARKVVHDYRQGIVPAGLTENELWR
  AKYAYDSAFHPDTGEKMTLIGRMSAQVPMNMTITGCMMTFYRTTPAVLFWQWINQSFNAVVNYTNRSGDA
  PLTVNELGTAYVSATTGAVATALGLNALTKRVSPLIGRFVPFAAVAAANCINIPLMRQRELKVGIPVTDE
  NGTRLGESTNAAKQAITQVVISRILMAAPGMAIPPFIMNTLEKKAFLKRFPWMSAPIQVTLVGFCLVFAT
  PLCCALFPQKSSMSVTSLEDELQASIQRTHPEIRRVYFNKGL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027324
• ORF Size: 966 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027324.5
• RefSeq Size: 2803 bp
• RefSeq ORF: 969 bp
• Locus ID: 14057
• UniProt ID: Q99JR1
• Cytogenetics: 13 28.4 cM
• Gene Summary: Mitochondrial serine transporter that mediates transport of serine into mitochondria, an important step of the one-carbon metabolism pathway (By similarity). Mitochondrial serine is converted to glycine and formate, which then exits to the cytosol where it is used to generate the charged folates that serve as one-carbon donors (By similarity). Transports both D-serine and L-serine (By similarity). Also able to transport other amino-acids, such as alanine (By similarity). May be indirectly involved in the transport of a component required for iron utilization into or out of the mitochondria (PubMed:11274051).[UniProtKB/Swiss-Prot Function]