Gtf2b (NM_145546) Mouse Tagged ORF Clone

CAT#: MG204535

Gtf2b (tGFP-tagged) - Mouse general transcription factor IIB (Gtf2b)

Product Images

Circular map for MG204535

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gtf2b
• Synonyms: MGC6859
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204535 representing NM_145546
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGACCAGCCGTTTGGATGCTCTCCCAAGAGTCACATGTCCGAATCATCCAGATGCAATTTTAG
  TTGAAGACTACAGAGCTGGCGATATGATCTGTCCCGAATGTGGCCTAGTTGTAGGGGACCGAGTTATTGA
  TGTGGGATCTGAATGGAGAACTTTCAGCAATGACAAAGCAACAAAAGACCCATCTAGAGTTGGAGACTCT
  CAGAATCCTCTTCTGAGTGATGGAGATTTGTCCACCATGATTGGCAAGGGTACAGGAGCTGCAAGTTTTG
  ATGAGTTTGGCAATTCTAAGTATCAGAACCGGAGAACAATGAGTAGCTCTGATCGAGCAATGATGAACGC
  ATTCAAGGAAATCACGACCATGGCGGACAGAATCAACCTCCCTCGCAATATAGTTGATCGAACAAATAAT
  TTATTCAAGCAAGTGTATGAGCAGAAGAGCCTGAAGGGCAGAGCTAATGACGCGATAGCCTCTGCTTGTC
  TGTACATCGCCTGTAGACAAGAAGGCGTTCCCAGGACATTTAAAGAAATATGTGCTGTGTCTCGAATCTC
  TAAGAAAGAAATTGGCCGCTGTTTTAAACTGATTTTGAAAGCTCTGGAAACCAGCGTGGATCTGATCACA
  ACTGGGGACTTCATGTCCAGGTTCTGCTCCAACCTTTGCCTCCCCAAGCAAGTGCAGATGGCAGCTACAC
  ACATAGCCCGCAAGGCAGTGGAGCTGGACTTGGTTCCTGGCAGGAGCCCGATCTCTGTGGCGGCAGCAGC
  TATTTACATGGCTTCCCAGGCTTCAGCTGAGAAGCGAACACAGAAAGAAATCGGGGATATTGCTGGTGTT
  GCTGATGTTACAATCCGACAGTCCTACAGACTGATCTACCCTCGGGCTCCGGATCTCTTCCCTTCAGACT
  TCAAGTTTGACACCCCAGTGGACAAATTACCCCAGCTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204535 representing NM_145546
  Red=Cloning site Green=Tags(s)
  
  MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDS
  QNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNN
  LFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLIT
  TGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGV
  ADVTIRQSYRLIYPRAPDLFPSDFKFDTPVDKLPQL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145546
• ORF Size: 948 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145546.1, NP_663521.1
• RefSeq Size: 1267 bp
• RefSeq ORF: 951 bp
• Locus ID: 229906
• UniProt ID: P62915
• Cytogenetics: 3 H1
• Gene Summary: General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element. Modulates transcription start site selection. Exhibits also autoacetyltransferase activity that contributes to the activated transcription.[UniProtKB/Swiss-Prot Function]