Olfr722 (NM_146494) Mouse Tagged ORF Clone

CAT#: MG204363

Olfr722 (tGFP-tagged) - Mouse olfactory receptor 722 (Olfr722)

Product Images

Circular map for MG204363

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Olfr722
• Synonyms: MOR241-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204363 representing NM_146494
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATCAAGAACAGCTCAGTAGTAACAGAATTCATCCTACTTGGCCTGACCCAGTCTCAAGAAGCTC
  AACTCCTGGTCTTCGCACTGATTTCAGTTTTCTACCTAATAATTCTCCCTGGAAATTTTCTTATCATTTT
  CACCATTAGATCAGACTCTGGACTCACAGCCCCACTTTACTTCTTTCTGGGAAACTTGGCATTCCTGGAT
  GCCTCCTACTCTTTCATTGTAGCTCCTAGAATGCTTGTGGATTTCTTCTGTGAGAAAAAAGTCATTTCTT
  ATAAAGCCTGCATCACTCAGCTATTCTTTCTGCACTTTCTTGGAGCAGGAGAAATGTTTCTTCTTGTTGT
  GATGGCCGTTGATCGTTATATCGCTATATGCCGTCCTTTATACTACTCCACTCTCATGAACCCTAGAGTC
  TGTTATGCCTTATTGTTGGCTCTGTGGCTTGGGGGTTTTGCTCATTCCATTGTACAAGTGGCTCTTATCC
  TAAACTTGCCCTTTTGTGGGCCAAACCAGCTGGATAACTTTTTCTGTGATGTTCCACAGGTTGTAAAGCT
  AGCCTGCACTGATACCTTTGCTGTGGAGCTCCTGATGGTTTCTAACAGTGGTCTACTTACCCTCTTATGC
  TTCTTGGGACTTCTGGCCTCCTATGCTGTCATTCTCTACCACGTAAAAGGACATTCTTCTGAAGGGAAGA
  GCAAAGCCATCTCTACATGCACCACCCATATTATAATTGTGTTTCTCATGTTTGGGCCTGCCATTTTTAT
  CTATACCCGCCCCTTCCAGGCTCTCCAAGCTGACAAAGTTGTTTCCCTTTTCCACACGGTCATCTTTCCT
  TTGATGAATCCTGTGATCTATACACTTCGCAACCAGGAAGTGAAAACTTCTATGAGGAAGTTGTTAAGTC
  AGTATGTGATTTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204363 representing NM_146494
  Red=Cloning site Green=Tags(s)
  
  MEIKNSSVVTEFILLGLTQSQEAQLLVFALISVFYLIILPGNFLIIFTIRSDSGLTAPLYFFLGNLAFLD
  ASYSFIVAPRMLVDFFCEKKVISYKACITQLFFLHFLGAGEMFLLVVMAVDRYIAICRPLYYSTLMNPRV
  CYALLLALWLGGFAHSIVQVALILNLPFCGPNQLDNFFCDVPQVVKLACTDTFAVELLMVSNSGLLTLLC
  FLGLLASYAVILYHVKGHSSEGKSKAISTCTTHIIIVFLMFGPAIFIYTRPFQALQADKVVSLFHTVIFP
  LMNPVIYTLRNQEVKTSMRKLLSQYVIC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146494
• ORF Size: 924 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146494.2, NP_666705.1
• RefSeq Size: 1655 bp
• RefSeq ORF: 927 bp
• Locus ID: 258487
• Cytogenetics: 14 C1
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]