Cope (NM_021538) Mouse Tagged ORF Clone

CAT#: MG204352

Cope (tGFP-tagged) - Mouse coatomer protein complex, subunit epsilon (Cope)

Product Images

Circular map for MG204352

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cope
• Synonyms: 1110005D17Rik; Cope1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204352 representing NM_021538
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCTCCGGTTCCTGGCGCGGTCTCTGGCGGCTCCGGAGAGGTAGATGAGCTGTTCGACGTGAAGA
  ACGCTTTCTACATCGGCAGCTACCAGCAGTGCATCAACGAGGCTCAGCGCGTGAAGCTCTCCAGTCCTGA
  GCGGGAAGTAGAGAGGGATGTCTTCCTATACAGAGCATACCTCGCACAGAGGAAGTATGGCGTGGTCCTG
  GATGAGATCAAACCCTCCTCGGCCCCAGAACTCCAGGCTGTGCGCATGTTTGCTGAGTACCTTGCCAGTG
  AGAACCAGAGGGACAGCATCGTGCTGGAGCTGGATCGGGAGATGAGCAGGAGTGTGGATGTGACCAATAC
  CACTTTCCTGCTCATGGCTGCCTCCATCTACTTCCACGACCAGAACCCGGATGCAGCCCTGCGAACCCTG
  CACCAGGGTGACGGCCTTGAGTGCATGGCCATGACGATTCAGATCCTCCTCAAGCTGGACAGGCTGGACC
  TAGCCCGGAAGGAGCTGAAGAAGATGCAGGACCAAGATGAGGACGCCACCCTTACCCAGCTAGCCACTGC
  CTGGGTCAACCTGGCTGTGGGTGGTGAGAAGCTACAAGAAGCCTACTACATATTCCAAGAGCTGGCCGAC
  AAGTGCTCCCCCACACTGCTGCTGCTCAATGGCCAGGCAGCCTGCCACTCGGCACAGGGCCGCTGGGAGA
  CTGCAGAGGGTGTGCTGCAAGAGGCACTGGACAAGGACAGCGGCCACCCTGAGACCCTCATCAATCTCAT
  TGTACTGTCACAGCACCTGGGCAAGCCCCCTGAGGTGACAAACCGATACTTGTCACAGCTGAAGGATGCA
  CACAGGGCCCACCCCTTCATCAAGGAGTACCAGGCCAAGGAGAACGATTTCGATCGCCTGGCAATGCAGT
  ATGCGCCCAGTGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204352 representing NM_021538
  Red=Cloning site Green=Tags(s)
  
  MAPPVPGAVSGGSGEVDELFDVKNAFYIGSYQQCINEAQRVKLSSPEREVERDVFLYRAYLAQRKYGVVL
  DEIKPSSAPELQAVRMFAEYLASENQRDSIVLELDREMSRSVDVTNTTFLLMAASIYFHDQNPDAALRTL
  HQGDGLECMAMTIQILLKLDRLDLARKELKKMQDQDEDATLTQLATAWVNLAVGGEKLQEAYYIFQELAD
  KCSPTLLLLNGQAACHSAQGRWETAEGVLQEALDKDSGHPETLINLIVLSQHLGKPPEVTNRYLSQLKDA
  HRAHPFIKEYQAKENDFDRLAMQYAPSA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021538
• ORF Size: 924 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021538.1, NP_067513.1
• RefSeq Size: 1043 bp
• RefSeq ORF: 927 bp
• Locus ID: 59042
• UniProt ID: O89079
• Cytogenetics: 8 B3.3
• Gene Summary: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).[UniProtKB/Swiss-Prot Function]