Cdk5r1 (BC050768) Mouse Tagged ORF Clone

CAT#: MG204342

Cdk5r1 (tGFP-tagged) - Mouse cyclin-dependent kinase 5, regulatory subunit (p35) 1 (cDNA clone MGC:58293 IMAGE:6533538)

Product Images

Circular map for MG204342

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cdk5r1
• Synonyms: p35, Cdk5r, p25
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204342 representing BC050768
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACGGTGCTGTCCCTATCCCCCAGCTATCGGAAGGCCACACTGTTTGAGGATGGCGCGGCCACCG
  TGGGCCACTACACGGCCGTGCAGAACAGCAAGAACGCCAAGGACAAGAACCTGAAGCGGCACTCCATCAT
  CTCGGTGCTGCCTTGGAAGAGGATCGTGGCGGTGTCAGCGAAGAAGAAGAACTCCAAGAAGGCGCAGCCC
  AACAGCAGCTACCAGAGCAACATCGCGCATCTCAACAATGAGAACCTGAAGAAGTCGCTGTCCTGTGCCA
  ACCTGTCCACATTCGCCCAGCCCCCGCCGGCTCAGCCGCCCGCACCCCCAGCCAGCCAGCTTTCTGGCTC
  CCAGACTGGGGTCTCCTCTTCTGTCAAGAAGGCCCCGCACCCTGCCATCACCTCTGCAGGGACACCCAAA
  CGGGTCATCGTCCAGGCGTCCACTAGTGAGCTGCTGCGCTGCCTGGGTGAGTTTCTCTGCCGCCGGTGCT
  ACCGCCTGAAGCACTTGTCCCCAACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCCCTGCTTCT
  GCAGGGCTGGCAGGACCAGGGTTTCATCACACCGGCCAATGTGGTCTTCCTCTACATGCTCTGCAGGGAT
  GTCATCTCCTCTGAGGTGGGCTCCGACCATGAGCTCCAGGCTGTCCTGCTGACCTGTCTGTACCTCTCCT
  ATTCCTACATGGGCAATGAGATCTCCTACCCGCTCAAGCCCTTCCTGGTAGAGAGCTGTAAGGAAGCCTT
  TTGGGACCGTTGCCTCTCAGTTATCAACCTCATGAGCTCCAAGATGCTGCAGATCAATGCCGACCCACAC
  TATTTCACACAAGTGTTCTCTGACTTGAAGAATGAGAGCGGTCAGGAGGACAAGAAGCGACTCCTCCTGG
  GGCTGGATCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204342 representing BC050768
  Red=Cloning site Green=Tags(s)
  
  MGTVLSLSPSYRKATLFEDGAATVGHYTAVQNSKNAKDKNLKRHSIISVLPWKRIVAVSAKKKNSKKAQP
  NSSYQSNIAHLNNENLKKSLSCANLSTFAQPPPAQPPAPPASQLSGSQTGVSSSVKKAPHPAITSAGTPK
  RVIVQASTSELLRCLGEFLCRRCYRLKHLSPTDPVLWLRSVDRSLLLQGWQDQGFITPANVVFLYMLCRD
  VISSEVGSDHELQAVLLTCLYLSYSYMGNEISYPLKPFLVESCKEAFWDRCLSVINLMSSKMLQINADPH
  YFTQVFSDLKNESGQEDKKRLLLGLDR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC050768
• ORF Size: 921 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050768, AAH50768
• RefSeq Size: 4166 bp
• RefSeq ORF: 923 bp
• Locus ID: 12569
• Cytogenetics: 11 47.94 cM
• Gene Summary: p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution.[UniProtKB/Swiss-Prot Function]