Tspan12 (NM_173007) Mouse Tagged ORF Clone

CAT#: MG204278

Tspan12 (tGFP-tagged) - Mouse tetraspanin 12 (Tspan12)

Product Images

Circular map for MG204278

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tspan12
• Synonyms: 9030619E17; AI426782; AI663988; AW111457; Tm4sf12
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204278 representing NM_173007
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGAGAAGATTCCGTGAAGTGTTTGCGCTGCTTGCTCTACGCCCTCAACCTGCTCTTTTGGTTAA
  TGTCCATCAGTGTCTTGGCAGTTTCTGCTTGGATGAGGGACTACCTGAATAATGTTTTGACTTTAACTGC
  AGAAACAAGGGTAGAAGAAGCGGTCATCTTAACTTACTTCCCCGTGGTTCACCCCGTCATGATTGCTGTC
  TGCTGCTTCCTTATCATCGTGGGAATGCTGGGATACTGTGGAACGGTGAAAAGAAATCTGTTGCTTCTTG
  CATGGTACTTCGGAACGTTACTCGTCATCTTCTGTGTAGAACTGGCTTGCGGTGTGTGGACATACGAGCA
  GGAGGTTATGGTGCCAGTACAGTGGTCAGATATGGTTACTTTGAAAGCCAGAATGACAAATTACGGCTTA
  CCGAGGTATAGATGGCTTACACACGCTTGGAATTATTTTCAGAGAGAGTTTAAGTGCTGTGGAGTGGTGT
  ACTTCACTGACTGGTTGGAAATGACAGAAATGGACTGGCCTCCCGACTCCTGCTGTGTCAGGGAGTTCCC
  AGGGTGCTCCAAGCAGGCTCACCAGGAAGATCTCAGTGACCTTTACCAAGAGGGTTGTGGGAAGAAGATG
  TATTCCTTTTTGAGAGGAACCAAACAATTGCAAGTTCTAAGGTTTCTGGGAATCTCCATTGGTGTGACAC
  AAATCCTGGCCATGATTCTCACCATTACTCTGCTCTGGGCTCTGTATTATGATAGAAGGGAGCCTGGAAC
  AGACCAAATGCTATCTCTGAAAAATGATACGTCTCAGCACTTGTCATGTCATTCTGTGGAACTGTTAAAA
  CCAAGTCTTTCAAGGATCTTTGAGCATACATCAATGGCAAACAGCTTCAATACACATTTTGAGATGGAAG
  AATTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204278 representing NM_173007
  Red=Cloning site Green=Tags(s)
  
  MAREDSVKCLRCLLYALNLLFWLMSISVLAVSAWMRDYLNNVLTLTAETRVEEAVILTYFPVVHPVMIAV
  CCFLIIVGMLGYCGTVKRNLLLLAWYFGTLLVIFCVELACGVWTYEQEVMVPVQWSDMVTLKARMTNYGL
  PRYRWLTHAWNYFQREFKCCGVVYFTDWLEMTEMDWPPDSCCVREFPGCSKQAHQEDLSDLYQEGCGKKM
  YSFLRGTKQLQVLRFLGISIGVTQILAMILTITLLWALYYDRREPGTDQMLSLKNDTSQHLSCHSVELLK
  PSLSRIFEHTSMANSFNTHFEMEEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173007
• ORF Size: 915 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173007.4
• RefSeq Size: 2491 bp
• RefSeq ORF: 918 bp
• Locus ID: 269831
• UniProt ID: Q8BKT6
• Cytogenetics: 6 A3.1
• Gene Summary: Regulator of cell surface receptor signal transduction. Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity (By similarity). Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1).[UniProtKB/Swiss-Prot Function]