Fhl1 (NM_001077362) Mouse Tagged ORF Clone

CAT#: MG204101

Fhl1 (tGFP-tagged) - Mouse four and a half LIM domains 1 (Fhl1), transcript variant 2

Product Images

Circular map for MG204101

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fhl1
• Synonyms: FHL-1; KyoT; RAM14-1; SLIM; SLIM-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204101 representing NM_001077362
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTCAAAGACACTCAGGTCCCTCCAGCTATAAGGTGGGCACCATGTCGGAGAAGTTCGACTGTC
  ACTACTGCAGGGACCCCTTGCAGGGGAAGAAGTACGTGCAGAAGGATGGCCGTCACTGCTGCCTGAAGTG
  CTTTGACAAGTTCTGCGCCAACACCTGCGTGGACTGCCGCAAGCCCATAAGCGCTGATGCCAAGGAGGTG
  CATTATAAGAATCGCTACTGGCATGACAACTGCTTCCGCTGTGCCAAGTGCCTTCACCCCTTGGCCAGTG
  AGACCTTTGTGTCCAAGGATGGCAAGATCCTGTGCAACAAGTGCGCTACTCGGGAGGACTCCCCCAGGTG
  CAAAGGGTGCTTCAAGGCCATTGTGGCAGGAGACCAGAATGTGGAGTACAAGGGCACCGTCTGGCATAAA
  GACTGCTTCACCTGCAGCAACTGCAAGCAAGTCATTGGGACCGGAAGCTTCTTCCCGAAAGGGGAGGACT
  TCTACTGTGTGACTTGCCATGAGACCAAGTTCGCCAAGCATTGCGTGAAGTGCAACAAGGCCATCACATC
  TGGAGGAATCACTTACCAGGATCAGCCCTGGCATGCCGAGTGCTTTGTGTGTGTTACCTGCTCTAAGAAG
  CTGGCTGGGCAGCGTTTCACCGCTGTGGAGGACCAGTATTACTGCGTGGATTGCTACAAGAACTTTGTGG
  CCAAGAAGTGTGCTGGATGCAAGAACCCCATCACTGGGTTTGGTAAAGGCTCCAGTGTGGTGGCCTATGA
  AGGACAATCCTGGCACGACTACTGCTTCCACTGCAAAAAATGCTCCGTGAATCTGGCCAACAAGCGCTTT
  GTATTTCATAATGAGCAGGTGTATTGCCCTGACTGTGCCAAAAAGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204101 representing NM_001077362
  Red=Cloning site Green=Tags(s)
  
  MASQRHSGPSSYKVGTMSEKFDCHYCRDPLQGKKYVQKDGRHCCLKCFDKFCANTCVDCRKPISADAKEV
  HYKNRYWHDNCFRCAKCLHPLASETFVSKDGKILCNKCATREDSPRCKGCFKAIVAGDQNVEYKGTVWHK
  DCFTCSNCKQVIGTGSFFPKGEDFYCVTCHETKFAKHCVKCNKAITSGGITYQDQPWHAECFVCVTCSKK
  LAGQRFTAVEDQYYCVDCYKNFVAKKCAGCKNPITGFGKGSSVVAYEGQSWHDYCFHCKKCSVNLANKRF
  VFHNEQVYCPDCAKKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077362
• ORF Size: 888 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077362.1, NP_001070830.1
• RefSeq Size: 2324 bp
• RefSeq ORF: 891 bp
• Locus ID: 14199
• UniProt ID: P97447
• Cytogenetics: X A6
• Gene Summary: May have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP-J and plays a negative regulatory role in the RBP-J-mediated transcription in mammalian systems.[UniProtKB/Swiss-Prot Function]