Sgf29 (NM_029339) Mouse Tagged ORF Clone

CAT#: MG204029

Ccdc101 (tGFP-tagged) - Mouse coiled-coil domain containing 101 (Ccdc101)

Product Images

Circular map for MG204029

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sgf29
• Synonyms: 1700023O11Rik; 9530025I05Rik; Ccdc101
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204029 representing NM_029339
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTTGTGTCTGCTGATTCTCGAATTGCAGAGCTTCTCACAGAGCTCCATCAGCTGATCAAGCAAA
  CCCAGGAAGAGCGGTCGAGAAGTGAGCACAACTTGGTGAACATCCAGAAAACCCACGAGCGGATGCAGAC
  AGAGAACAAGATTTCTCCTTATTACCGGACAAAGCTTCGAGGCCTCTACACAACCGCCAAGACTGATGCG
  GAGGCCGAGTGCAACATCCTCCGCAAAGCGCTGGATAAGATAGCTGAGATCAAGTCTCTGTTGGAAGAGA
  GGCGGATTGCGGCCAAGATCGCAGGTCTCTACAATGACTCAGAGCCCCCACGGAAGACCATGCGCAGAGG
  GGTGCTGATGACACTGCTGCAGCAGTCCGCCATGACCCTGCCCCTCTGGATCGGGAAACCTGGTGACAAG
  CCCCCACCCCTCTGTGGAGCCATTCCAGCCTCAGGGGACTATGTGGCCAAACCTGGAGACAAGGTGGCTG
  CTAGGGTGAAGGCTGTGGAAGGGGATGAACAGTGGATCCTAGCTGAAGTAGTCAGTTACAGCCATGCTAC
  CAACAAGTATGAGGTAGACGACATTGATGAAGAAGGCAAAGAGAGACACACCCTGAGCCGTCGGCGCATC
  ATCCCACTGCCCCAGTGGAAAGCTAACCCTGAGACAGACCCTGAGGCCTTGTTCCAGAAGGACCAGCTGG
  TGCTGGCCCTATATCCCCAGACCACCTGCTTCTACCGTGCCCTGATCCACACCCCACCACAGAGGCCTCA
  GGATGACTACTCAGTCCTGTTTGAAGACACCTCTTATGCAGATGGCTACTCCCCTCCCCTCAATGTGGCC
  CAGAGGTACGTGGTGGCTTGTAAGGAGCCCAAGAAAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204029 representing NM_029339
  Red=Cloning site Green=Tags(s)
  
  MALVSADSRIAELLTELHQLIKQTQEERSRSEHNLVNIQKTHERMQTENKISPYYRTKLRGLYTTAKTDA
  EAECNILRKALDKIAEIKSLLEERRIAAKIAGLYNDSEPPRKTMRRGVLMTLLQQSAMTLPLWIGKPGDK
  PPPLCGAIPASGDYVAKPGDKVAARVKAVEGDEQWILAEVVSYSHATNKYEVDDIDEEGKERHTLSRRRI
  IPLPQWKANPETDPEALFQKDQLVLALYPQTTCFYRALIHTPPQRPQDDYSVLFEDTSYADGYSPPLNVA
  QRYVVACKEPKKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029339
• ORF Size: 879 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029339.2, NP_083615.2
• RefSeq Size: 1140 bp
• RefSeq ORF: 882 bp
• Locus ID: 75565
• UniProt ID: Q9DA08
• Cytogenetics: 7 F3
• Gene Summary: Chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes like the TFTC-HAT, ATAC or STAGA complexes. SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3). In the SAGA-type complexes, SGF29 is required to recruit complexes to H3K4me. Involved in the response to endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival.[UniProtKB/Swiss-Prot Function]