Morf4l2 (BC088731) Mouse Tagged ORF Clone

CAT#: MG203932

Morf4l2 (tGFP-tagged) - Mouse mortality factor 4 like 2 (cDNA clone MGC:91037 IMAGE:5340043)

Product Images

Circular map for MG203932

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Morf4l2
• Synonyms: Sid393p, MRGX, mKIAA0026
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203932 representing BC088731
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCCAGAAAGCAGGCTTCTCAAACTCGTGGACAACAATCTGCTGAAGAAGACAACTTTAAGAAAC
  CAACTCGAAGCAATATGCAGAGAAGTAAGATGAGAGGAGCTGCCTCGGGAAAGAAGTCAGCTGGTTCGCA
  GCCAAAGAATCTCGATCCAGCCCTGCCCGGAAGATGGGGAGGTCGCTCTGCTGAGAACCCCCCTTCCGGT
  TCTGTGCGGAAGACCAGGAAGAACAAGCAGAAGGCTCCTGGCAACGGAGACGGAGGCAGTACCAGTGAAG
  TCCCCCAGCCCCCTCGGAAGAAAAGGGCACGGGCTGACCCCACTGTGGAGAGCGAGGAGGCATTCAAGAG
  TAGGATGGAGGTGAAGGTGAAGATCCCTGAAGAATTAAAACCGTGGCTGGTGGAGGACTGGGACTTGGTT
  ACGAGGCAGAAGCAGTTGTTCCAGCTCCCTGCTAAAAAGAATGTCGATGCCATTCTTGAGGAGTATGCCA
  ATTGTAAGAAGTCGCAGGGAAATGTTGATAATAAGGAGTACGCAGTTAATGAAGTTGTAGGAGGGATAAA
  AGAGTATTTCAATGTGATGCTGGGCACTCAGCTGCTGTACAAGTTTGAAAGGCCTCAGTATGCTGAGATT
  CTGCTGGCTCACCCTGATGCGCCGATGTCGCAGATCTATGGGGCGCCACACCTCCTGAGATTATTCGTGA
  GAATTGGGGCAATGTTGGCCTATACGCCCCTTGATGAGAAAAGCCTGGCATTATTGCTGGGCTATCTGCA
  TGATTTCCTTAAGTATCTGGCAAAGAATTCTGCCTCTCTGTTTACTGCCAGTGATTACAAAGTGGCTTCT
  GCTGACTATCATCGCAAAGCCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203932 representing BC088731
  Red=Cloning site Green=Tags(s)
  
  MSSRKQASQTRGQQSAEEDNFKKPTRSNMQRSKMRGAASGKKSAGSQPKNLDPALPGRWGGRSAENPPSG
  SVRKTRKNKQKAPGNGDGGSTSEVPQPPRKKRARADPTVESEEAFKSRMEVKVKIPEELKPWLVEDWDLV
  TRQKQLFQLPAKKNVDAILEEYANCKKSQGNVDNKEYAVNEVVGGIKEYFNVMLGTQLLYKFERPQYAEI
  LLAHPDAPMSQIYGAPHLLRLFVRIGAMLAYTPLDEKSLALLLGYLHDFLKYLAKNSASLFTASDYKVAS
  ADYHRKAL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC088731
• ORF Size: 866 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC088731, AAH88731
• RefSeq Size: 1582 bp
• RefSeq ORF: 866 bp
• Locus ID: 56397
• Cytogenetics: X F1
• Gene Summary: Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the MSIN3A complex which acts to repress transcription by deacetylation of nucleosomal histones (By similarity).[UniProtKB/Swiss-Prot Function]