Capza2 (NM_007604) Mouse Tagged ORF Clone
CAT#: MG203894
- TrueORF®
Capza2 (tGFP-tagged) - Mouse capping protein (actin filament) muscle Z-line, alpha 2 (Capza2)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_007604" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Capza2 |
Synonyms | 1110053K06Rik; AW208808; Cappa2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203894 representing NM_007604
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATCTGGAGGAGCAGTTGTCCGATGAGGAGAAGGTTCGTATAGCAGCAAAATTCATCATTCACG CCCCTCCTGGAGAATTTAATGAGGTTTTTAATGATGTTCGATTATTGCTTAATAATGATAATCTTCTCAG GGAAGGAGCCGCACATGCATTTGCACAGTATAACCTGGACCAGTTTACACCAGTAAAAATTGAAGGCTAT GAAGATCAGGTTTTGATAACAGAACATGGAGACTTGGGAAATGGAAAATTTTTGGATCCAAAAAACAGAA TCTGTTTCAAATTTGATCATTTAAGAAAGGAAGCAACTGATCCAAGGCCCTACGAAGCAGAAAATGCCAT TGAATCCTGGAGAACTTCAGTAGAAACTGCACTGCGAGCTTATGTAAAAGAGCATTATCCGAATGGGGTC TGCACTGTGTATGGCAAAAAAGTAGATGGGCAGCAAACCATTATTGCATGCATAGAAAGCCATCAGTTTC AAGCAAAAAACTTTTGGAATGGTCGTTGGAGGTCAGAGTGGAAGTTTACAGTCACTCCTTCCACCACACA GGTGGTTGGCATTTTGAAAATTCAGGTTCATTATTATGAAGATGGTAATGTTCAGCTAGTGAGTCATAAA GATATACAAGATTCCCTCACAGTATCTAATGAGGTGCAAACAGCAAAAGAATTTATAAAGATTGTAGAAG CTGCAGAAAATGAATACCAGACTGCCATCAGTGAGAATTATCAGACGATGTCGGACACTACCTTCAAAGC CTTACGTCGACAGTTGCCAGTGACACGTACTAAGATCGACTGGAACAAGATCCTTAGCTACAAGATTGGC AAAGAGATGCAGAATGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203894 representing NM_007604
Red=Cloning site Green=Tags(s) MADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGY EDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPYEAENAIESWRTSVETALRAYVKEHYPNGV CTVYGKKVDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTVTPSTTQVVGILKIQVHYYEDGNVQLVSHK DIQDSLTVSNEVQTAKEFIKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIG KEMQNA TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_007604 |
ORF Size | 858 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007604.3 |
RefSeq Size | 2015 bp |
RefSeq ORF | 861 bp |
Locus ID | 12343 |
UniProt ID | P47754 |
Cytogenetics | 6 7.96 cM |
Gene Summary | F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203429 | Capza2 (untagged) - Mouse capping protein (actin filament) muscle Z-line, alpha 2 (Capza2), (10ug) |
USD 300.00 |
|
MR203894 | Capza2 (Myc-DDK-tagged) - Mouse capping protein (actin filament) muscle Z-line, alpha 2 (Capza2) |
USD 450.00 |
|
MR203894L3 | Lenti ORF clone of Capza2 (Myc-DDK-tagged) - Mouse capping protein (actin filament) muscle Z-line, alpha 2 (Capza2) |
USD 750.00 |
|
MR203894L4 | Lenti ORF clone of Capza2 (mGFP-tagged) - Mouse capping protein (actin filament) muscle Z-line, alpha 2 (Capza2) |
USD 750.00 |
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