Agpat1 (BC009651) Mouse Tagged ORF Clone
CAT#: MG203879
- TrueORF®
Agpat1 (tGFP-tagged) - Mouse 1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, alpha) (cDNA clone
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Agpat1 |
Synonyms | 1-AGPAT, 1-AGP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203879 representing BC009651
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGTGGCCCGGGGCCTGGACGGCGCTGCTGCAGCTGCTGCTCCTGCTGCTGTCCACCCTGTGGT TCTGCAGCTCCAGTGCCAAGTATTTCTTTAAGATGGCCTTCTACAACGGCTGGATCCTCTTCCTGGCGAT CCTCGCAATCCCCGTGTGTGCTGTGCGAGGGCGCAATGTCGAGAACATGAAGATCTTGCGTCTGCTGCTG CTCCACGTCAAATACCTGTATGGGATCCGAGTGGAGGTGCGCGGGGCTCACCACTTCCCTCCCACGCAGC CCTACGTGGTTGTGTCCAACCACCAGAGTTCCCTCGACCTGCTTGGAATGATGGAGGTCCTGCCAGATCG TTGTGTGCCCATTGCCAAGCGTGAGCTACTATGGGCTGGCTCTGCTGGGCTGGCCTGCTGGCTGGCAGGA ATCATCTTCATTGACCGGAAACGCACAGGGGACGCCATCAGTGTCATGTCCGAGGTGGCTCAGACCTTGC TCACCCAGGATGTGAGAGTCTGGGTTTTTCCTGAAGGAACAAGAAACCACAATGGCTCCATGCTGCCCTT CAAACGAGGCGCCTTCCACCTTGCAGTACAGGCCCAGGTCCCCATCATCCCCATCGTCATGTCCTCCTAT CAAGACTTCTACTCCAAAAAGGAAAGACGCTTCACCTCGCCTGGACGTTGTCAGGTGCGGGTGCTGCCTC CAGTGTCCACAGAAGGGCTGACACCAGATGACGTCCCAGCTCTGGCAGACAGCGTCCGGCACTCCATGCT CACCATCTTCCGGGAGATCTCCACTGACGGCCTGGGTGGTGGCGACTGTCTGAAGAAGCCTGGGGGAGCG GGCGAAGCCCGGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203879 representing BC009651
Red=Cloning site Green=Tags(s) MELWPGAWTALLQLLLLLLSTLWFCSSSAKYFFKMAFYNGWILFLAILAIPVCAVRGRNVENMKILRLLL LHVKYLYGIRVEVRGAHHFPPTQPYVVVSNHQSSLDLLGMMEVLPDRCVPIAKRELLWAGSAGLACWLAG IIFIDRKRTGDAISVMSEVAQTLLTQDVRVWVFPEGTRNHNGSMLPFKRGAFHLAVQAQVPIIPIVMSSY QDFYSKKERRFTSPGRCQVRVLPPVSTEGLTPDDVPALADSVRHSMLTIFREISTDGLGGGDCLKKPGGA GEARL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC009651 |
ORF Size | 857 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC009651, AAH09651 |
RefSeq Size | 2048 bp |
RefSeq ORF | 857 bp |
Locus ID | 55979 |
Cytogenetics | 17 18.18 cM |
Gene Summary | Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.[UniProtKB/Swiss-Prot Function] |
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