Icmt (NM_133788) Mouse Tagged ORF Clone
CAT#: MG203862
- TrueORF®
Icmt (tGFP-tagged) - Mouse isoprenylcysteine carboxyl methyltransferase (Icmt)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_133788" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Icmt |
Synonyms | 1700008E11Rik; C80758; Gm13095; HSTE14; OTTMUSG00000010406; pcCMT; PPMT; STE14 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203862 representing NM_133788
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCTGCGCGGCGCGGGTTCCGCCGGGCTCCGAGGCGCGCCTCAGCCTCGCTACATTCCTCCTGG GCGCCTCGGTGCTCGCTCTGCCGCTGCTCACGCGCGCCGGCCTGCAGGGCCGCACCGGGCTGGCGCTCTA CGTGGCCGGACTCAACGCGCTGCTGCTGCTACTCTACCGGCCGCCGCGCTACCAGATAGCCATCAGAGCT TGTTTCCTTGGCTTTGTGTTTGGCTGTGGTGTGCTGCTAAGTTTCAGCCAGTCCTCTTGGAATCACTTTG GCTGGTACGTGTGCTCGCTGTCTCTGTTCCACTATTCTGAGTACTTAGTGACAGCTGTCAATAACCCCAA AAGTCTGTCCCTGGACTCCTTCCTCCTGAATCACAGCCTGGAGTACACGGTAGCTGCCCTGTCTTCTTGG ATAGAGTTCACGCTCGAGAACATCTTCTGGCCAGAACTGAAGCAGATTACCTGGCTGAGTGCCACCGGGC TCCTGATGGTGGTCTTCGGAGAATGCCTGAGGAAAGCTGCCATGTTTACGGCTGGCTCCAACTTCAACCA TGTGGTGCAGAGTGAAAAGTCAGACACCCACACTCTGGTGACAAGTGGGGTGTATGCTTGGTGCCGGCAC CCCTCCTATGTGGGCTGGTTTTACTGGAGTATCGGAACCCAGGTGATGCTGTGTAATCCCATCTGTGGCG TGGTCTATGCCCTGACGGTGTGGCGCTTCTTCCGAGATCGCACAGAAGAGGAGGAGATCTCACTGATTCA CTTTTTTGGGGAAGAGTACTTGGATTATAAAAAGAGGGTGCCCACAGGCCTGCCTTTCATAAAGGGGGTC AAGGTGGAGCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203862 representing NM_133788
Red=Cloning site Green=Tags(s) MAGCAARVPPGSEARLSLATFLLGASVLALPLLTRAGLQGRTGLALYVAGLNALLLLLYRPPRYQIAIRA CFLGFVFGCGVLLSFSQSSWNHFGWYVCSLSLFHYSEYLVTAVNNPKSLSLDSFLLNHSLEYTVAALSSW IEFTLENIFWPELKQITWLSATGLLMVVFGECLRKAAMFTAGSNFNHVVQSEKSDTHTLVTSGVYAWCRH PSYVGWFYWSIGTQVMLCNPICGVVYALTVWRFFRDRTEEEEISLIHFFGEEYLDYKKRVPTGLPFIKGV KVEL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_133788 |
ORF Size | 852 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133788.2, NP_598549.1 |
RefSeq Size | 4796 bp |
RefSeq ORF | 855 bp |
Locus ID | 57295 |
UniProt ID | Q9EQK7 |
Cytogenetics | 4 E2 |
Gene Summary | Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207537 | Icmt (untagged) - Mouse isoprenylcysteine carboxyl methyltransferase (Icmt), transcript variant 1, (10ug) |
USD 330.00 |
|
MR203862 | Icmt (Myc-DDK-tagged) - Mouse isoprenylcysteine carboxyl methyltransferase (Icmt), transcript variant 1 |
USD 300.00 |
|
MR203862L3 | Lenti ORF clone of Icmt (Myc-DDK-tagged) - Mouse isoprenylcysteine carboxyl methyltransferase (Icmt), transcript variant 1 |
USD 600.00 |
|
MR203862L4 | Lenti ORF clone of Icmt (mGFP-tagged) - Mouse isoprenylcysteine carboxyl methyltransferase (Icmt), transcript variant 1 |
USD 600.00 |
{0} Product Review(s)
Be the first one to submit a review