Nsmce1 (NM_026330) Mouse Tagged ORF Clone

CAT#: MG203781

Nsmce1 (tGFP-tagged) - Mouse non-SMC element 1 homolog (S. cerevisiae) (Nsmce1)

Product Images

Circular map for MG203781

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nsmce1
• Synonyms: 2510027N19Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203781 representing NM_026330
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCGGCGTGATCTCGCTGGTTTGCGCTCCCTCCAACATGCAGGGCAGCACGAGGAGAGCAGGAG
  CCATGACGGATGTGCACCGGCGCTTCCTCCAGCTGCTCATGACCCACGGTGTGCTGGAGGAGTGGGAGGT
  TCGGCGTCTGCAGAACCACTGCTACCAGGTCCATGACCGAAATGCGACTGTAGATAAGCTGGAGGACTTC
  ATCAACAACATCAACAGTGTCCTGGAGTCTCTGTATATTGAGATAAAGAAAGGAGTCACGGAAGATGATG
  GGAGACCCATTTATGCACTGGTGAATCTTGCTACAACTTCAGTTTCCAAAATGGCTACGGATTTTGCAGA
  GAACGAGCTGGATCTGTTTAGAAAGGCTCTGGAACTGATCGTTGACTCAGAAACTGGCTTTGCTTCTTCT
  ACAAACATTTTGAACCTAGTTGATCAACTCAAAGGGAAAAAGATGAGGAAGAAGGAGGCCGAGCAGGTGC
  TGCAGAAGTTTGTGCAGAGCAAGTGGTTGATTGAGAAGGAGGGAGAGTTCACCTTGCATGGCCGGGCTAT
  CTTGGAGATGGAGCAGTTCATCCGAGAGAGCTACCCAGATTCTGTGAAGATGTGCAACATCTGCCACGGC
  CTCCTCATCCAGGGTCAAAGTTGTGAGACGTGTGGAATTAGGATGCATTTGCCTTGTGTGGCCAAATACT
  TCCAGTCCATCCCTGAACCACACTGCCCCCACTGTAATGACTACTGGCCCCACGATATACCAGAAGTCTA
  CAACCCTGAGAAGGAGAGGGAAGCTGGCATCTCCAAGTCGAGCAGAAAGTCCTTACGGACCCGGCAGCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203781 representing NM_026330
  Red=Cloning site Green=Tags(s)
  
  MASGVISLVCAPSNMQGSTRRAGAMTDVHRRFLQLLMTHGVLEEWEVRRLQNHCYQVHDRNATVDKLEDF
  INNINSVLESLYIEIKKGVTEDDGRPIYALVNLATTSVSKMATDFAENELDLFRKALELIVDSETGFASS
  TNILNLVDQLKGKKMRKKEAEQVLQKFVQSKWLIEKEGEFTLHGRAILEMEQFIRESYPDSVKMCNICHG
  LLIQGQSCETCGIRMHLPCVAKYFQSIPEPHCPHCNDYWPHDIPEVYNPEKEREAGISKSSRKSLRTRQH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026330
• ORF Size: 840 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026330.3
• RefSeq Size: 1120 bp
• RefSeq ORF: 843 bp
• Locus ID: 67711
• UniProt ID: Q9D720
• Cytogenetics: 7 F3
• Gene Summary: RING-type zinc finger-containing E3 ubiquitin ligase that assembles with melanoma antigen protein (MAGE) to catalyze the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. Within MAGE-RING ubiquitin ligase complex, MAGE stimulates and specifies ubiquitin ligase activity likely through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. Involved in maintenance of genome integrity, DNA damage response and DNA repair. NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair.[UniProtKB/Swiss-Prot Function]