Ube2s (NM_133777) Mouse Tagged ORF Clone

CAT#: MG202594

Ube2s (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2S (Ube2s)

Product Images

Circular map for MG202594

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ube2s
• Synonyms: 0910001J09Rik; 6720465F12Rik; AA409170; E2-EPF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202594 representing NM_133777
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCCAATGTGGAGAATCTGCCGCCCCACATCATCCGCCTAGTGTACAAGGAGGTGACAACACTGA
  CGGCAGACCCACCTGATGGCATTAAAGTCTTCCCCAATGAGGAGGATCTCACAGACCTGCAGGTTACCAT
  CGAGGGCCCTGAAGGGACTCCCTATGCTGGAGGTCTGTTCCGTATGAAGCTCCTACTGGGGAAGGACTTC
  CCTGCCTCCCCACCCAAGGGCTACTTCCTGACTAAAATCTTCCACCCAAATGTGGGCCCCAATGGCGAGA
  TCTGTGTCAATGTGCTCAAGAGGGACTGGACGGCTGAATTGGGCATACGACATGTGCTGCTGACCATCAA
  GTGCCTGCTGATCCACCCTAACCCAGAGTCTGCACTCAATGAGGAGGCAGGCCGCCTGCTTTTGGAGAAT
  TATGAAGAGTATGCTGCCCGGGCCCGTCTGCTCACAGAAATCCATGGGGGTGCATGCAGCACCAGCAGCG
  GGAGGGCTGAGGCTACCCAAGACCTGGCCAGTGGGGCTTCAGCCTCCTCTGCTGACCCTATGATCCCAGG
  GGTCCTAGGGGGAGCTGAGGGTCCCATGGCCAAGAAACATGCAGGTGAGCGAGATAAGAAGCTGGCAGCC
  AAGAAAAAGTTGGACAAGAAGCGAGCACTGAGGCGACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202594 representing NM_133777
  Red=Cloning site Green=Tags(s)
  
  MNSNVENLPPHIIRLVYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDF
  PASPPKGYFLTKIFHPNVGPNGEICVNVLKRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLEN
  YEEYAARARLLTEIHGGACSTSSGRAEATQDLASGASASSADPMIPGVLGGAEGPMAKKHAGERDKKLAA
  KKKLDKKRALRRL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133777
• ORF Size: 669 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133777.2, NP_598538.1
• RefSeq Size: 988 bp
• RefSeq ORF: 672 bp
• Locus ID: 77891
• UniProt ID: Q921J4
• Cytogenetics: 7 A1
• Gene Summary: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.[UniProtKB/Swiss-Prot Function]