Scn1b (NM_011322) Mouse Tagged ORF Clone
CAT#: MG202467
- TrueORF®
Scn1b (tGFP-tagged) - Mouse sodium channel, voltage-gated, type I, beta (Scn1b)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_011322" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Scn1b |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG202467 representing NM_011322
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACGCTGCTGGCTCTCGTGGTGGGCGCGGCGCTGGTATCCTCAGCCTGGGGGGGCTGCGTGGAGG TGGATTCCGATACCGAGGCTGTGTATGGGATGACCTTCAAAATCCTGTGCATCTCCTGTAAGCGTCGTAG TGAGACCACCGCCGAAACCTTCACGGAGTGGACCTTCCGCCAGAAGGGGACAGAGGAATTTGTCAAGATC CTACGCTATGAGAATGAGGTGCTGCAGCTGGAGGAAGATGAGCGCTTTGAGGGCCGAGTGGTGTGGAACG GTAGTCGGGGCACCAAGGACCTGCAGGACCTGTCCATCTTCATCACCAACGTCACCTACAACCACTCTGG CGACTACGAATGTCACGTCTACCGTCTCCTCTTCTTTGATAATTATGAGCACAACACCAGCGTCGTCAAG AAGATCCACCTGGAGGTGGTGGACAAGGCCAACAGAGATATGGCATCCATCGTGTCAGAGATCATGATGT ACGTGCTCATTGTGGTGTTGACCATATGGCTTGTGGCAGAGATGGTGTACTGCTACAAGAAGATTGCTGC TGCTACGGAGGCTGCTGCACAAGAGAACGCCTCAGAATACCTGGCCATTACATCCGAGAGCAAAGAAAAC TGTACAGGCGTCCAGGTGGCTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202467 representing NM_011322
Red=Cloning site Green=Tags(s) MGTLLALVVGAALVSSAWGGCVEVDSDTEAVYGMTFKILCISCKRRSETTAETFTEWTFRQKGTEEFVKI LRYENEVLQLEEDERFEGRVVWNGSRGTKDLQDLSIFITNVTYNHSGDYECHVYRLLFFDNYEHNTSVVK KIHLEVVDKANRDMASIVSEIMMYVLIVVLTIWLVAEMVYCYKKIAAATEAAAQENASEYLAITSESKEN CTGVQVAE TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_011322 |
ORF Size | 654 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011322.3 |
RefSeq Size | 1568 bp |
RefSeq ORF | 657 bp |
Locus ID | 20266 |
UniProt ID | P97952 |
Cytogenetics | 7 19.3 cM |
Gene Summary | Regulatory subunit of multiple voltage-gated sodium channel complexes that play important roles in excitable membranes in brain, heart and skeletal muscle. Enhances the presence of the pore-forming alpha subunit at the cell surface and modulates channel gating characteristics and the rate of channel inactivation. Modulates the activity of a variety of pore-forming alpha subunits, such as SCN1A, SCN2A, SCN3A, SCN4A, SCN5A and SCN10A.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207389 | Scn1b (untagged) - Mouse sodium channel, voltage-gated, type I, beta (Scn1b), (10ug) |
USD 330.00 |
|
MR202467 | Scn1b (Myc-DDK-tagged) - Mouse sodium channel, voltage-gated, type I, beta (Scn1b) |
USD 300.00 |
|
MR202467L3 | Lenti ORF clone of Scn1b (Myc-DDK-tagged) - Mouse sodium channel, voltage-gated, type I, beta (Scn1b) |
USD 600.00 |
|
MR202467L4 | Lenti ORF clone of Scn1b (mGFP-tagged) - Mouse sodium channel, voltage-gated, type I, beta (Scn1b) |
USD 600.00 |
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