Vti1a (NM_016862) Mouse Tagged ORF Clone

CAT#: MG202429

Vti1a (tGFP-tagged) - Mouse vesicle transport through interaction with t-SNAREs homolog 1A (yeast) (Vti1a)

Product Images

Circular map for MG202429

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Vti1a
• Synonyms: 1110014F16Rik; 1110018K19Rik; 4921537J05Rik; MVti1; MVti1a; Vti1; Vti1-rp2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202429 representing NM_016862
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCCGACTTCGAAGGGTACGAGCAGGACTTCGCAGTGCTCACTGCTGAGATCACCAGCAAGATTG
  CAAGGGTCCCTCGGCTCCCTCCCGATGAGAAGAAGCAAATGGTTGCCAATGTGGAGAAACAGCTTGAAGA
  GGCCAGAGAACTGCTTGAACAGATGGATTTGGAAGTTCGAGAAATTCCACCCCAAAGTCGAGGAATGTAT
  AGCAACAGGATGAGAAGCTACAAGCAAGAAATGGGGAAACTGGAAACGGATTTTAAAAGGTCACGGATTG
  CCTACAGTGACGAAGTACGGAATGAGCTCCTAGGGGATGCTGGGAATTCCTCCGAGAACCAGAGGGCACA
  TCTGCTGGATAACACGGAGAGGCTGGAAAGGTCGTCTCGGAGACTAGAGGCTGGGTACCAGATAGCAGTG
  GAGACCGAGCAAATCGGCCAAGAGATGTTGGAAAACCTGAGTCATGACAGAGAAAAGATACAGCGGGCAC
  GTGACCGACTCCGGGACGCAGACGCAAACCTGGGGAAGAGCTCTCGGATTCTGACGGGGATGCTGCGAAG
  AATCATCCAGAACCGTATCCTGCTCGTCATCTTGGGGATCATCGTGGTCATCGCCATCCTGACGGCCATC
  GCGTTTTTCGTCAAAGGACAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202429 representing NM_016862
  Red=Cloning site Green=Tags(s)
  
  MSSDFEGYEQDFAVLTAEITSKIARVPRLPPDEKKQMVANVEKQLEEARELLEQMDLEVREIPPQSRGMY
  SNRMRSYKQEMGKLETDFKRSRIAYSDEVRNELLGDAGNSSENQRAHLLDNTERLERSSRRLEAGYQIAV
  ETEQIGQEMLENLSHDREKIQRARDRLRDADANLGKSSRILTGMLRRIIQNRILLVILGIIVVIAILTAI
  AFFVKGH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016862
• ORF Size: 651 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016862.2, NP_058558.1
• RefSeq Size: 3406 bp
• RefSeq ORF: 654 bp
• Locus ID: 53611
• UniProt ID: O89116
• Cytogenetics: 19 D2
• Gene Summary: V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be concerned with increased secretion of cytokines associated with cellular senescence.[UniProtKB/Swiss-Prot Function]