Xrcc3 (BC043073) Mouse Tagged ORF Clone

CAT#: MG202365

Xrcc3 (tGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (cDNA clone MGC:57978 IMAGE:6404521)

Product Images

Circular map for MG202365

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Xrcc3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202365 representing BC043073
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTTGGATCAGCTGGACCTAAACCCCCGAATTACTGCTGCGGTTAAGAGGGGCAGACTGAAGTCAG
  TGAAGGAGATTCTGTGCTACTCCGGACCAGACTTGCAGCGGCTCACCGGCCTGCCCAGCCACGATGTGCA
  GTGCCTGCTGAGAGCTGCCTCTCTACACCTTCGGGGAAGCCGCGTCCTCTCAGCACTGCATCTGTTCCAG
  CAGAAGGAGAGCTTCCCTGAGCAGCATCAACGCCTGAGCCTAGGCTGCCCTGTCCTGGATCAGTTCCTGG
  GTGGAGGCCTGCCCCTGGAGGGCATCACTGGCCTGGCTGGCTGCAGCTCAGCAGGAAAGACCCAGCTGGC
  GCTACAGCTCTGCCTGGCTGTGCAGTTCCCAAGACAATATGGAGGCCTAGAGGCCGGGGCTGTCTACATC
  TGCACAGAAGATGCCTTCCCCAGCAAGCGGCTGTGGCAGCTCATCGCGCAGCAGCGGAGGCTGCGGACAG
  ACGCGCCTGAGGAGCTGATCGAGAAGATCAGGTTCAGCAACCACATCTTCATTGAACACGCGGCCGACGT
  GCCCCATTTCGTTGTGAGTTCCACCTTCAGGCCTCAGCCATCAGGGCGAAGCTCCTGCTCTCGCTGGGGG
  CCACACTGCGAAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202365 representing BC043073
  Red=Cloning site Green=Tags(s)
  
  MDLDQLDLNPRITAAVKRGRLKSVKEILCYSGPDLQRLTGLPSHDVQCLLRAASLHLRGSRVLSALHLFQ
  QKESFPEQHQRLSLGCPVLDQFLGGGLPLEGITGLAGCSSAGKTQLALQLCLAVQFPRQYGGLEAGAVYI
  CTEDAFPSKRLWQLIAQQRRLRTDAPEELIEKIRFSNHIFIEHAADVPHFVVSSTFRPQPSGRSSCSRWG
  PHCED
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC043073
• ORF Size: 647 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC043073, AAH43073
• RefSeq Size: 2318 bp
• RefSeq ORF: 647 bp
• Locus ID: 74335
• Cytogenetics: 12 F1
• Gene Summary: This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. Allelic variants in the human gene are associated with susceptibility to breast cancer and cutaneous malignant melanoma. [provided by RefSeq, Sep 2015]