Rgs4 (NM_009062) Mouse Tagged ORF Clone

CAT#: MG202147

Rgs4 (tGFP-tagged) - Mouse regulator of G-protein signaling 4 (Rgs4)

Product Images

Circular map for MG202147

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rgs4
• Synonyms: AA004315; AA597169; ESTM48; ESTM50
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202147 representing NM_009062
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCAAAGGACTTGCAGGTCTGCCGGCTTCCTGCCTGAGGAGTGCAAAGGACATGAAACATCGGCTGG
  GGTTCCTGCTGCAGAAGTCAGATTCCTGCGAACACAGTTCTTCACACAGCAAGAAGGACAAAGTAGTAAC
  TTGCCAGAGGGTAAGCCAAGAAGAAGTCAAGAAATGGGCTGAATCGTTGGAAAACCTGATTCACCATGAA
  TGTGGACTGGCAGCTTTCAAAGCTTTCCTGAAGTCGGAATACAGCGAGGAGAACATTGACTTCTGGATCA
  GCTGTGAGGAGTACAAGAAGATCAAGTCACCTTCTAAACTAAGTCCCAAAGCCAAGAAGATCTACAACGA
  GTTCATCTCAGTGCAAGCAACAAAAGAGGTGAACCTGGACTCTTGCACCAGAGAGGAGACAAGCCGGAAC
  ATGTTACAGCCCACAATAACCTGTTTTGATGAAGCCCAGAAGAAGATTTTCAACCTGATGGAAAAGGATT
  CCTACCGCCGCTTCCTCAAGTCTCGATTCTATCTTGACCTGACCAATCCTTCCAGCTGCGGGGCAGAGAA
  GCAGAAAGGAGCCAAGAGTTCTGCAGACTGCACTTCCCTGGTCTCCCAGTGTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202147 representing NM_009062
  Red=Cloning site Green=Tags(s)
  
  MCKGLAGLPASCLRSAKDMKHRLGFLLQKSDSCEHSSSHSKKDKVVTCQRVSQEEVKKWAESLENLIHHE
  CGLAAFKAFLKSEYSEENIDFWISCEEYKKIKSPSKLSPKAKKIYNEFISVQATKEVNLDSCTREETSRN
  MLQPTITCFDEAQKKIFNLMEKDSYRRFLKSRFYLDLTNPSSCGAEKQKGAKSSADCTSLVSQCA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009062
• ORF Size: 615 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009062.3
• RefSeq Size: 2781 bp
• RefSeq ORF: 618 bp
• Locus ID: 19736
• UniProt ID: O08899
• Cytogenetics: 1 76.84 cM
• Gene Summary: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein (By similarity).[UniProtKB/Swiss-Prot Function]