Zmat2 (NM_025594) Mouse Tagged ORF Clone

CAT#: MG201998

Zmat2 (tGFP-tagged) - Mouse zinc finger, matrin type 2 (Zmat2)

Product Images

Circular map for MG201998

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Zmat2
• Synonyms: 2610510D14Rik; 2900082I05Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201998 representing NM_025594
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCTGGCAGCGGGACAAAAAACTTGGACTTTCGCCGAAAGTGGGACAAAGATGAATATGAGAAGC
  TCGCGGAGAAGAGACTCACAGAGGAGAGAGAGAAGAAGGATGGGAAACCAGTGCAGCCTGTCAAGCGGGA
  GCTTCTGCGGCACAGGGATTACAAGGTGGACCTGGAGTCCAAGCTGGGGAAGACGATTGTTATCACCAAG
  ACGACCCCACAGTCTGAAATGGGGGGCTACTACTGCAATGTCTGTGACTGTGTGGTGAAAGACTCCATTA
  ACTTCCTGGACCACATTAATGGAAAGAAACATCAGCGAAACCTGGGTATGTCCATGCGCGTGGAACGCTC
  CACTCTGGATCAGGTAAAGAAGCGTTTTGAGGTGAACAAGAAGAAGATGGAAGAGAAGCAGAAAGATTAT
  GATTTTGAGGAAAGGATGAAGGAGCTCAGAGAAGAGGAGGAAAAGGCTAAAGCATACAAAAAAGAGAAAC
  AGAAGGAGAAGAAAAGGAGAGCCGAGGAGGACTTGACTTTTGAGGAGGATGATGAAATGGCAGCTGTGAT
  GGGTTTCTCTGGCTTTGGTTCCACCAAGAAGAGTTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201998 representing NM_025594
  Red=Cloning site Green=Tags(s)
  
  MASGSGTKNLDFRRKWDKDEYEKLAEKRLTEEREKKDGKPVQPVKRELLRHRDYKVDLESKLGKTIVITK
  TTPQSEMGGYYCNVCDCVVKDSINFLDHINGKKHQRNLGMSMRVERSTLDQVKKRFEVNKKKMEEKQKDY
  DFEERMKELREEEEKAKAYKKEKQKEKKRRAEEDLTFEEDDEMAAVMGFSGFGSTKKSY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025594
• ORF Size: 597 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025594.2
• RefSeq Size: 2257 bp
• RefSeq ORF: 600 bp
• Locus ID: 66492
• UniProt ID: Q9CPW7
• Cytogenetics: 18 B2
• Gene Summary: Involved in pre-mRNA splicing as a component of the spliceosome.[UniProtKB/Swiss-Prot Function]