Itpa (NM_025922) Mouse Tagged ORF Clone

CAT#: MG201990

Itpa (tGFP-tagged) - Mouse inosine triphosphatase (nucleoside triphosphate pyrophosphatase) (Itpa)

Product Images

Circular map for MG201990

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Itpa
• Synonyms: 2010016I08Rik; AU020102; Itp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201990 representing NM_025922
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGTCTTTGGTCGGGAAGAAGATCGTGTTTGTGACGGGAAACGCCAAGAAGCTGGAGGAGGTCA
  TTCAGATTCTCGGAGATAATTTTCCATGCACTTTGGAGGCTCAGAAAATTGACCTGCCTGAGTACCAGGG
  AGAACCGGATGAGATTTCCATACAGAAGTGTCGGGAGGCAGCTCGACAGGTGCAGGGCCCTGTCCTGGTG
  GAAGATACCTGTCTGTGCTTTAACGCACTTGGGGGACTGCCTGGCCCCTACATAAAATGGTTCCTACAGA
  AGCTGAAGCCTGAAGGTCTCCACCAGCTCCTGGCCGGCTTTGAAGACAAATCGGCCTATGCACTCTGCAC
  ATTCGCTCTCAGCACTGGGGACCCAAGCCAGCCAGTGCTTCTCTTCAGAGGCCAGACCTCGGGACAGATT
  GTGATGCCACGAGGCAGCCGGGACTTTGGCTGGGATCCCTGCTTTCAGCCTGATGGATATGAGCAAACGT
  ATGCAGAGATGCCAAAGTCTGAGAAGAACACCATTTCTCATCGGTTCCGGGCCCTGCACAAGCTACAGGA
  GTACTTTAGTGTGGCTGCTGGGGCTGGTGACCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201990 representing NM_025922
  Red=Cloning site Green=Tags(s)
  
  MAASLVGKKIVFVTGNAKKLEEVIQILGDNFPCTLEAQKIDLPEYQGEPDEISIQKCREAARQVQGPVLV
  EDTCLCFNALGGLPGPYIKWFLQKLKPEGLHQLLAGFEDKSAYALCTFALSTGDPSQPVLLFRGQTSGQI
  VMPRGSRDFGWDPCFQPDGYEQTYAEMPKSEKNTISHRFRALHKLQEYFSVAAGAGDH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025922
• ORF Size: 594 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025922.3
• RefSeq Size: 1264 bp
• RefSeq ORF: 597 bp
• Locus ID: 16434
• UniProt ID: Q9D892
• Cytogenetics: 2 63.24 cM
• Gene Summary: Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.[UniProtKB/Swiss-Prot Function]