Cabp5 (NM_013877) Mouse Tagged ORF Clone

CAT#: MG201486

Cabp5 (tGFP-tagged) - Mouse calcium binding protein 5 (Cabp5)

Product Images

Circular map for MG201486

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cabp5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201486 representing NM_013877
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTTTCCAATGGGTCCTGCCTGCATCTTCTTGAGAAAAGGCATTGCTGAAAAGCAGCGGGAGAGAC
  CACTGGGACAGGATGAGCTTGATGAGCTCCGGGAAGCATTTCTTGAATTTGACAAGGACCAAGATGGGTT
  CATCTCTTACAAGGATTTGGGCAATCTCATGAGAACGATGGGTTACATGCCTACAGAGATGGAGTTGACT
  GAGTTGGGCCAGCAAATCCGAATGAACCTTGGTGGCCGTGTAGACTTTGAAGACTTTGTGGAACTGATGA
  CCCCCAAATTGCTTGCAGAGACAGCAGGGATGATTGGTGTCCAGGAGATGCGAGATGCCTTCAAGGAGTT
  TGATGCCAATGGAGATGGGGAGATCACACTGGCAGAACTGCAGCAGGCCATGCAGAGGCTGCTGGGGGAG
  AAGCTCACACCCCGGGAGATTGCTGAGGTGGTACAGGAGGCTGATATCAATGGAGATGGCACTGTTGACT
  TTGAAGAGTTTGTGAAGATGATGTCTCGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201486 representing NM_013877
  Red=Cloning site Green=Tags(s)
  
  MQFPMGPACIFLRKGIAEKQRERPLGQDELDELREAFLEFDKDQDGFISYKDLGNLMRTMGYMPTEMELT
  ELGQQIRMNLGGRVDFEDFVELMTPKLLAETAGMIGVQEMRDAFKEFDANGDGEITLAELQQAMQRLLGE
  KLTPREIAEVVQEADINGDGTVDFEEFVKMMSR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013877
• ORF Size: 519 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013877.3, NP_038905.1
• RefSeq Size: 1746 bp
• RefSeq ORF: 522 bp
• Locus ID: 29865
• UniProt ID: Q9JLK3
• Cytogenetics: 7 A1
• Gene Summary: Inhibits calcium-dependent inactivation of L-type calcium channel and shifts voltage dependence of activation to more depolarized membrane potentials (PubMed:18586882). Involved in the transmission of light signals (PubMed:18586882). May positively regulate neurotransmitter vesicle endocytosis and exocytosis in a salt-dependent manner (PubMed:22039235). May play a role in the extension and network organization of neurites (PubMed:22039235).[UniProtKB/Swiss-Prot Function]