Pla2g2c (NM_008868) Mouse Tagged ORF Clone

CAT#: MG201069

Pla2g2c (tGFP-tagged) - Mouse phospholipase A2, group IIC (Pla2g2c)

Product Images

Circular map for MG201069

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pla2g2c
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201069 representing NM_008868
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGCATTGCTGTCTTCCTTGTCTTCATCTTCTACTGGACAACCTCCACCCTCAGCAGTTTCTGGC
  AGTTCCAGAGGATGGTCAAACACGTCACAGGGCGCAGCGCCTTCTTCTCCTATTACGGATATGGCTGCTA
  CTGTGGGCTTGGGGGCAAAGGGCTCCCTGTGGATGCCACAGACAGGTGCTGCTGGGCTCATGACTGCTGC
  TACCACAAGCTTAAGGAATATGGCTGCCAACCCATCTTGAATGCCTACCAGTTCACCATTGTCAATGGGA
  CGGTGACCTGTGGATGCACCGTGGCCAGCAGCTGCCTCTGCGGGCAGAAGGCCTGTGAGTGTGACAAACA
  GTCTGTGTACTGCTTCAAGGAGAACCTGGCCACCTACGAGAAGGCCTTCAAGCAGCTCTTCCCGACCAGG
  CCTCAGTGTGGCAGGGACAAACTCCAGTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201069 representing NM_008868
  Red=Cloning site Green=Tags(s)
  
  MKGIAVFLVFIFYWTTSTLSSFWQFQRMVKHVTGRSAFFSYYGYGCYCGLGGKGLPVDATDRCCWAHDCC
  YHKLKEYGCQPILNAYQFTIVNGTVTCGCTVASSCLCGQKACECDKQSVYCFKENLATYEKAFKQLFPTR
  PQCGRDKLQC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008868
• ORF Size: 450 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008868.1, NP_032894.1
• RefSeq Size: 1445 bp
• RefSeq ORF: 453 bp
• Locus ID: 18781
• UniProt ID: P48076
• Cytogenetics: 4 70.54 cM
• Gene Summary: PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Testis PA2 may be important in the production of prostaglandins, by the release of arachidonic acid, which in turn are necessary for the contractions of the seminiferous tubules and the testicular capsule; they also seem to decrease sperm transit time through the male reproductive tract.[UniProtKB/Swiss-Prot Function]