Bcas2 (BC013627) Mouse Tagged ORF Clone

CAT#: MG200878

Bcas2 (tGFP-tagged) - Mouse breast carcinoma amplified sequence 2 (cDNA clone MGC:7712 IMAGE:3497838)

Product Images

Circular map for MG200878

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bcas2
• Synonyms: MGC7712
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200878 representing BC013627
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCACGGGCTTGGTAGCCGGAGAGGTGGTGGTAGATGCGCTACCATATTTTGACCAAGGCTATG
  AGGCACCCGGCGTGCGGGAAGCGGCGGCGGCCCTGGTGGAGGAGGAGACGCGCAGATACCGACCTACCAA
  GAACTACCTGAGCTACCTGACGGCCCCGGATTATTCTGCCTTTGAAACAGACATAATGAGAAATGAATTT
  GAAAGACTCGCTGCTCGACAACCGATTGAATTACTCAGCATGAAACGATATGAACTTCCAGCCCCTTCCT
  CAGGTCAAAAAAATGACATTACTGCATGGCAAGAATGTGTAAACAATTCTATGGCTCAGTTGGAGCACCA
  GGCGGTCCGGATCGAGAATCTGGAGCTGATGTCACAGCATGGATGCAATGCCTGGAAGGTGTACAATGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200878 representing BC013627
  Red=Cloning site Green=Tags(s)
  
  MAGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYRPTKNYLSYLTAPDYSAFETDIMRNEF
  ERLAARQPIELLSMKRYELPAPSSGQKNDITAWQECVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC013627
• ORF Size: 422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013627
• RefSeq Size: 3659 bp
• RefSeq ORF: 422 bp
• Locus ID: 68183
• Cytogenetics: 3 F2.2
• Gene Summary: Required for pre-mRNA splicing as component of the activated spliceosome. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).[UniProtKB/Swiss-Prot Function]