Eppin (NM_029325) Mouse Tagged ORF Clone

CAT#: MG200789

Eppin (tGFP-tagged) - Mouse serine protease inhibitor-like, with Kunitz and WAP domains 1 (eppin) (Spinlw1)

Product Images

Circular map for MG200789

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Eppin
• Synonyms: 1700024E17Rik; Spinlw1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200789 representing NM_029325
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTTTCTGGATTTGTGAGCATTCTTGTGCTATTTGGCCTGCTTGCAAGAGTCCAGGGACCTAGTC
  TAGCTGACTTGCTGTTTCCCAGGAGATGCCCCAGATTCAGAGAGGAGTGTGAACACCAAGAGAGGGACCT
  TTGTACCAGGGACAGGGACTGCCCGAAGAAAGAGAAGTGCTGTGTCTTCAACTGTGGAAAGAAATGCTTA
  AACCCCCAACAAGATATCTGCAGCCTGCCGAAAGACTCCGGCTACTGTATGGCTTACTTTCGTCGTTGGT
  GGTTTAATAAAGAAAATAGCACGTGCCAAGTCTTCATCTATGGTGGCTGCCAAGGAAACAATAACAACTT
  CCAATCCCAAAGTATATGCCAGAACGCCTGCGAAAAAAAAAGCAGTCTCACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200789 representing NM_029325
  Red=Cloning site Green=Tags(s)
  
  MKLSGFVSILVLFGLLARVQGPSLADLLFPRRCPRFREECEHQERDLCTRDRDCPKKEKCCVFNCGKKCL
  NPQQDICSLPKDSGYCMAYFRRWWFNKENSTCQVFIYGGCQGNNNNFQSQSICQNACEKKSSLT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029325
• ORF Size: 402 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029325.2, NP_083601.1
• RefSeq Size: 755 bp
• RefSeq ORF: 405 bp
• Locus ID: 75526
• UniProt ID: Q9DA01
• Cytogenetics: 2 H3
• Gene Summary: Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility (By similarity).[UniProtKB/Swiss-Prot Function]