Cby1 (NM_028634) Mouse Tagged ORF Clone

CAT#: MG200689

Cby1 (tGFP-tagged) - Mouse PKD2 interactor, golgi and endoplasmic reticulum associated 1 (Pgea1)

Product Images

Circular map for MG200689

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cby1
• Synonyms: 1110014P06Rik; arb1; CBY; Pgea1; PGEA14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200689 representing NM_028634
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCTCTTTGGCAGCATATTCAGCCCAAAGAAGACCCCTCCTCGGAAGTCGGCCTCTCTCTCCAACC
  TACATTCTTTAGATCGGTCAACGCGGGAACTGGAGCTGGGGCTCGACTATGGAACTCCTACCATGAACCT
  GGCGGGGCAGAGCCTGAAGTTTGAAAACGGCCAGTGGGTGGCAGACTCCGTGATTAGTGGGGGTGTGGAC
  CGGAGGGAAACTCAGCGCCTGCGCAAACGAAACCAACAGTTAGAAGAAGAAAACAATCTCCTGCGGCTGA
  AAGTGGACATTCTGCTGGACATGCTTTCAGAAACCACTGCTGAGTCCCACCTAAAGGACAAAGAGCTGGA
  TGAACTGAAGGTTACCAACCGCAGGAGGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200689 representing NM_028634
  Red=Cloning site Green=Tags(s)
  
  MPLFGSIFSPKKTPPRKSASLSNLHSLDRSTRELELGLDYGTPTMNLAGQSLKFENGQWVADSVISGGVD
  RRETQRLRKRNQQLEEENNLLRLKVDILLDMLSETTAESHLKDKELDELKVTNRRRK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028634
• ORF Size: 381 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028634.3, NP_082910.1
• RefSeq Size: 1119 bp
• RefSeq ORF: 384 bp
• Locus ID: 73739
• UniProt ID: Q9D1C2
• Cytogenetics: 15
• Gene Summary: Inhibits the Wnt/Wingless pathway by binding to CTNNB1/beta-catenin and inhibiting beta-catenin-mediated transcriptional activation through competition with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin-2/PKD2 and possibly of other intracellular proteins. Promotes adipocyte and cardiomyocyte differentiation.[UniProtKB/Swiss-Prot Function]