Pyurf (NM_025574) Mouse Tagged ORF Clone
CAT#: MG200443
- TrueORF®
Pyurf (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class Y (Pigy)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_025574" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Pyurf |
Synonyms | 2610022G08Rik; AI847956; Prey |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200443 representing NM_025574
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAGCGCCACGTGCCGCAGGCTCGCCCCGGCGCTGCGGAGGCTCCGCGCACTGTCTGCAGTCGCCG GGAGGTTTCTGCAAGTGCCCGGGGCCAGGCTCTGCTCTGACCAGAGCGAGAGGGCTGAGCAGCCCCACAC CTTCCACCCGGCGCTGCTGCAGTTCCTGGTGTGTCCGCTCTCCAAGAAGCCGCTTAGATATGAAGCATCG ACAAATGAATTGGTTAATGAAGAGTTGGGAATAGCATATCCAATCATTGATGGGATCCCTAATATGATAC CACAGGCAGCAAGGACCACACGTCAAAATGAGAAGCAAGAAGAAGCTGAGCAACCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200443 representing NM_025574
Red=Cloning site Green=Tags(s) MLSATCRRLAPALRRLRALSAVAGRFLQVPGARLCSDQSERAEQPHTFHPALLQFLVCPLSKKPLRYEAS TNELVNEELGIAYPIIDGIPNMIPQAARTTRQNEKQEEAEQP TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025574 |
ORF Size | 336 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025574.3, NP_079850.1 |
RefSeq Size | 5410 bp |
RefSeq ORF | 339 bp |
Locus ID | 66459 |
UniProt ID | Q9D1C3 |
Cytogenetics | 6 B3 |
Gene Summary | This gene encodes a small protein with a conserved DUF343 domain. The human ortholog of this gene expresses two distinct proteins from upstream and downstream coding regions. The upstream CDS encoding a DUF343 domain-containing protein has been conserved at this mouse locus, but the downstream CDS encoding a subunit of an enzyme involved in glycosylphosphatidylinositol biosynthesis has not been conserved. Instead, a separate locus on mouse chromosome 9 encodes the mouse homolog of the human phosphatidylinositol glycan anchor biosynthesis, class Y protein. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202444 | Pyurf (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class Y (Pigy), nuclear gene encoding mitochondrial protein, (10ug) |
USD 150.00 |
|
MR200443 | Pyurf (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class Y (Pigy), nuclear gene encoding mitochondrial protein |
USD 150.00 |
|
MR200443L3 | Lenti ORF clone of Pyurf (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class Y (Pigy), nuclear gene encoding mitochondrial protein |
USD 450.00 |
|
MR200443L4 | Lenti ORF clone of Pyurf (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class Y (Pigy), nuclear gene encoding mitochondrial protein |
USD 450.00 |
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