Pmf1 (BC037139) Mouse Untagged Clone

CAT#: MC206732

Pmf1 (untagged) - Mouse polyamine-modulated factor 1 (cDNA clone MGC:46783 IMAGE:4952550), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pmf1
• Synonyms: 2600009M07Rik; AL033286; AW060657
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC037139
  CCACGCGTCCGTTTCAACATGGCTGAGGTGAGCCGAGATAGCGAGGCTGCGGAAAGGGGGCCTGAGGGCT CCTCTCCGGAAGCTGTGCCAGGGGACGCGACCATCCCCAGGGTGAAACTCCTGGACGCCATAGTAGACAC TTTCCTCCAGAAGCTAGTCGCCGACAGGAGCTACGAGAGGTTCACCACCTGCTACAAACACTTCCACCAG TTGAACCCTGAGGTGACGCAGAGGATCTATGACAAGTTTGTGGCTCAGTTGCAGACATCCATCCGCGAGG AAATCTCAGAAATCAAAGAGGAGGGGAACCTAGAAGCTGTCCTGAACTCCCTGGATAAGATCATAGAAGA AGGCAGAGAGCGCGGAGAGCCAGCCTGGCGACCCAGTGGAATCCCAGAGAAAGACCTGTGTAGTGTCATG GCACCCTACTTCCTGAAGCAACAGGATACCCTGTGTCATCAAGTACGGAAACAGGAAGCCAAGAACCAGG AACTGGCCGACGCTGTCCTGGCCGGGCGCAGGCAGGTGGAGGAGCTGCAGCAGCAGGTTCGGGCCCTCCA GCAAACATGGCAGGCTCTACACAGAGAGCAGAGGGAGCTGCTGTCAGTGCTGAGGGCGCCTGAGTGAACA GCTGCTGGGCTCAGCCCTCTAGAGCGCCTGTGGAGGCAGTTTCCAGGCAGTAATGGAGTCCCCACCACCG CTTAGGTTCTGCTGGGGTCACACTGCCTTCAGATTCCTGCCGCCATCTTCTGCCTGCAGGATTGGGACTC CCACCCTGGCATCAGACACCAGGCTTTGTCTTCTGGAGTTGCAGCCAGCGCTGCCCCATGCCCTTCTGAT CCTCCCCCTCTCCCACCGCCCTCCCTGGATAGGATTTGGGCAGGGTTTCAGTCTCCACTGATTGCCGCCT TGTTCTCCCTGACTGATTGCTGCCTTGCTGGCTAGCCCAAGGGCCCTGCCATGTTCTCCCCACATCCGTA AATAAACTTCCTTTCCCACACAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC037139
• Insert Size: 609 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC037139, AAH37139
• RefSeq Size: 1016 bp
• RefSeq ORF: 609 bp
• Locus ID: 67037
• Cytogenetics: 3 F1
• Gene Summary: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis (By similarity). May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.[UniProtKB/Swiss-Prot Function]