Eya1 (BC060260) Mouse Untagged Clone

CAT#: MC206678

Eya1 (untagged) - Mouse eyes absent 1 homolog (Drosophila) (cDNA clone MGC:69623 IMAGE:6848408), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eya1
• Synonyms: bor
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC060260
  AACCAATAAGGTTAGGACAAGAGACTAGCTGTGGTTTGCGTTGCAAAAACAAAACAAAACAAAAAGCCCC GAGGCTTCGTGGGCAGACCTACAAGGCTGCGCAAACAAATCGGGGGATGAGATTTTGCTGTTTCTTTGTC TAGGCGTCTCAGATGCTATCTGCCGCTGCCGTTCGCTGGGGCGCAAAGCCGTTCCCAAACAGACCGCGGC GAGCTGATGGCTCCGAGCTTGCGGCGAGGTAGCGAGTGTGCAGGGCCACTTCCGACTGGAAGCCTTCCTG TGGCGGTGCGCGGTGGCGGGGTCCTTCCTGGGGAAGGCGATCGCGCTCAGTCGCTCCACAGCCCGAGCCT GCAGCAGCGCCCGGGCGCAGTCCCCGGGAGCCGGGCAATCCCCGGCCTCTGCGCTCCGCTGCCAGCCGCG TCACAGCGCCTCTGAGAGCTCCCGGGTCCCCGTGTTCCTGCACAGCAGCCAAACTATGTCAGGAATGGAG GTCTGCTAACACAGAAAACTCCAAGGAGCACACTAAACCGGTGGAGGCAGCAGATGTGAGCGCTGTGCGA ACGTCTCACAGCAGTTCCGATGTTGCTCTTTCCTCAAGTTGCAGGTCTATGGAAATGCAGGATCTAACCA GCCCGCATAGCCGACTGAGTGGTAGTAGCGAATCCCCCAGTGGTCCCAAACTCGATAGCTCTCATATAAA TAGTACTTCCATGACTCCCAATGGCACCGAAGTTAAAACAGAGCCAATGAGCAGCAGTGAAATAGCTTCA ACAGCAGCAGACGGGTCTTTAGACAGTTTCTCAGGTTCAGCTCTCGGAAGCAGCAGCTTTAGTCCAAGAC CAGCTCACCCGTTCTCTCCACCACAGATTTATCCTTCCAACAGATCATACCCACATATTCTCCCTACCCC TTCCTCACAAACTATGGCTGCATATGGGCAAACACAGTTTACCACAGGAATGCAACAAGCCACAGCCTAC GCCACGTACCCACAGCCTGGACAGCCCTATGGAATTTCCTCCTATGGCATCAAGACGGAAAGTGGATTGT CACAGTCTCAGTCACCTGGACAGACGGGATTTCTTAGCTATGGCACAAGCTTTGGTACCCCTCAACCTGG ACAGGCACCGTACAGCTACCAGATGCAAGGTAGCAGCTTTACCACGTCATCAGGATTATATTCAGGAAAT AATTCACTCACCAACTCCTCCGGATTCAACAGTTCACAGCAGGACTATCCGTCTTATCCCGGCTTTGGCC AGGGTCAGTACGCACAGTATTATAACAGCTCGCCGTATCCAGCACACTACATGACGAGCAGTAACACCAG CCCGACCACACCGTCCACCAATGCCACTTACCAACTCCAGGAACCACCTTCTGGCGTCACAAGTCAGGCG GTCACAGACCCCACAGCAGAGTACAGTACAATCCACAGTCCTTCCACACCCATTAAAGAGACTGACTCCG AGCGGCTGCGTCGAGGTTCAGATGGGAAGTCACGTGGCCGAGGCAGAAGAAACAATAATCCCTCCCCTCC CCCGGATTCTGACCTTGAGAGAGTGTTCATCTGGGACCTGGACGAGACCATCATTGTTTTCCACTCCTTG CTCACGGGGTCCTACGCCAACAGATACGGGAGGGATCCACCTACTTCTGTTTCCCTGGGACTACGAATGG AAGAGATGATTTTCAACTTGGCAGACACACATCTATTTTTCAATGACCTAGAAGAGTGTGACCAAGTCCA TATAGATGATGTTTCATCAGACGACAACGGCCAGGACCTGAGCACATACAACTTTGGAACAGATGGCTTT CCTGCTGCAGCCACCAGTGCTAATTTATGCCTGGCAACTGGTGTCCGAGGTGGTGTGGACTGGATGCGGA AACTGGCCTTCCGCTACAGACGAGTAAAAGAGATCTACAACACCTACAAAAACAACGTGGGAGGTCTGCT TGGCCCAGCTAAGAGGGAAGCCTGGCTCCAGCTGAGGGCTGAGATTGAGGCACTCACAGACTCCTGGCTG ACCCTGGCCCTGAAGGCCCTCTCCCTCATCCACTCCCGGACGAACTGTGTGAATATTTTAGTAACAACTA CGCAGCTCATCCCAGCATTGGCAAAAGTCCTGCTATATGGATTAGGAATTGTGTTTCCAATAGAAAATAT TTACAGTGCAACTAAAATAGGAAAGGAAAGCTGTTTTGAGAGGATAATCCAAAGGTTTGGAAGGAAAGTG GTATACGTTGTCATAGGAGATGGTGTGGAAGAAGAGCAAGGGGCAAAAAAGCATGCTATGCCCTTCTGGA GGGTCTCCAGTCACTCGGACCTCATGGCACTGCATCATGCCTTGGAATTAGAGTACCTGTAACAGCTTCC TGCCAACTTGACACTGCACAACTGCCCTGTGGCCAGAGATAACCCAGCAGGCTTGTCTTCTTGTGTCAGT GCTGGACTTCAGGATGTACCAATTTCAGCATATGGATGCATAGCTGCTGCGGGCTTGACTGCTACCCTCG GGGTTAATGGAGGACCATGTGTATTCTTCAGAACAGCTGTTGACTCTAGTACTGTGAATCCAGTGAAAGT AAGCCATGAGAATGTTCTCACACAGTGTGGTGTGTCTTGGCTACATTAACTACATGGTTCAAACCTGTGA AGAAAAGACCTACAGACACTTTGGTTTTCGGCAAATGTGATACAAACAGCGTCTGTGATCCAGCAAACTT GATCCCACAACAAAGACTCCCACGCGTCACCTGGACAACCATGGAGCTATTTTATTTTCTCAGAGGTTCA CTTTTTGGTGTCCCCCACCCAGGGCAATCTGTACTTCGCTACTTATTTATGAAAAAAGACTTTGAAAAAA CAGATTTGCTGAGGTATAATTCATATACCATATGATTCACCAAGACAGACTTCTCGGCTTCAAATAGTTG AAGGGACAATAGCATAAGAAATAATCAATAAAAGGCCTTGGCCTGACAGTACAGCAAGTACTTTGAATTT TAGCACATTGCAAAAGTAGTTTAAAGTATGTCTAATTTAAATGTGTAAAATGTACATCACTGAGACAATC GTGTACAGAAGGAATTTTTGGTGTAAATTTGTAATAATGGATGATTCTTACACATTCTGTTTAGGGAATG CTATTATGAGGTAGAAATGCTTTCCTAGGAAGCTTGGGCACAAACTCCTGCAGTGCCTTATCTAAGGATT GGGTATAAATATTGTAGATAATGGATCTTTTTGATAATGTTATCTAAGACCAAAAGCATGAATGTCAAGT GTCAACAAGGATTTTATTTTCTTAGTCTTTTATCCCATTCATTTTTCTAATTTGTGTACGTGAGTGTGTG TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATTGGTTAGTTAGAAGGGACTTGATAAAAATAACAGT GGCTCACAACTTAAAAGTTTTGAAGTCTTCCTGACCACCTCCCCGACTTTCTCATTTCAACACTAATGTA TTATATGTAACTACTTGGAAAAGAATGACTATTCAAACACTTCATCCCACAGAAAGAATATAGATGATAG ATATATTTTATTAATAAAATTAACTGGAGCCTAGCAGAGTCTCCATGCACTCAGAATGATTACCGTGTCT GGGATTCTTGTTTATATCAACGAGGACTTTACTACATAACTGGGTCAGCAGCACTTCTCATCCCATCTCT TACTCTTTCCTTCCCACCCAGACAAGGTTCATTGCCCGATGGTGGGTTGGTCTGGCCATCATCAGAAACA CTAATGTTGCTCTCTACGTATGACAAGACAGACATAAACCTAGAGGGTGATGGGAGAAACACAAAAATGA TCATGTTTGTCATGTTATAAAAAAGATTCTTCCAAACTGATGAATTTGTATACGGTCAGGAGTCTTTCTC ATTGTGGTCTTTGTGTTGCCATAAAATTACCCTTTGGATCAATTTTAGTTTATATTATTAAGTTATTAAT AATATTATTTAATACTGGGGGCAGCAGGTTTGACCCAGAGTAGCAGAAAAACCACCCTACTTTTTTCTAT TCTAAAAGTCCATGTTGCTGCTAAAATTAGTCGTTATAAATTCTCTAAATTGTTAAAAATGAATAATGGA ATTAGTTTCTCCCCTGATGCAGACAGAAATGGCCAGTGTTGAGACCTCTTCATTTGATGCTGTTGTATTC TGCAGACTGAAAAGACAGCCTTGACCTTGGCCTTACTGTAAACAGGTGTGCTGTGTCCACGATTGTGTAC AGAACTTTTTTTTTCTTCATTAATGTTGTGTTTAAATTAATAAACTTGATTTGTGCAGTAAAAAAAAAAA AAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC060260
• Insert Size: 1758 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC060260, AAH60260
• RefSeq Size: 4354 bp
• RefSeq ORF: 1758 bp
• Locus ID: 14048
• Cytogenetics: 1 4.31 cM
• Gene Summary: Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5 (PubMed:10490620). Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress (PubMed:19234442). Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis (PubMed:14628042). Has also phosphatase activity with proteins phosphorylated on Ser and Thr residues (in vitro). Required for normal embryonic development of the craniofacial and trunk skeleton, kidneys and ears (PubMed:10471511). Together with SIX1, it plays an important role in hypaxial muscle development; in this it is functionally redundant with EYA2 (PubMed:17098221).[UniProtKB/Swiss-Prot Function]