Rad1 (BC048693) Mouse Untagged Clone

CAT#: MC206655

Rad1 (untagged) - Mouse RAD1 homolog (S. pombe) (cDNA clone MGC:59326 IMAGE:6508142), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rad1
• Synonyms: mRAD1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC048693
  CGGTGGCCTAGGTTTTCACACATCTTCCGGAAGAGGAGCATCCTTCTTCTCGGAGCCGACGGTACCGTGG ACCATCCATGCCTCTCCTAACCCAGTACAATGAAGAGGAGTACGAACAGTACTGCTTAGTGGCCAGCCTT GACAACGTTAGGAATCTCTCCACTGTCTTGAAAGCCATTCATTTCAGAGAACACGCCACGTGTTTTGCTA CCAAAAACGGAATCAAGGTTACAGTGGAGAATGCAAAGTGTGTGCAAGCAAATGCCTTTATTCAGGCTGA CGTGTTTCAGGAATTTGTCATTCAGGAAGAATCTGTTACTTTTCGAATTAACTTAACTATCCTTTTAGAC TGTTTATCTATTTTTGGATCAAGTCCTACACCAGGGACTTTGACTGCGCTTCGGATGTGTTACCAAGGTT ATGGTCACCCACTGATGCTATTTCTAGAAGAAGGAGGAGTGGTGACGGTCTGCAAAATTACCACTCAGGA GCCTGAGGAGACACTGGATTTTGATTTCTGCAGCACCAATGTTATGAATAAAATTATCCTGCAGTCAGAG GGGCTCCGGGAAGCCTTTTCTGAGCTGGACATGACAGGTGATGTCCTACAGATCACTGTGTCTCCTGACA AGCCCTATTTCAGGTTGTCTACTTTTGGAAATGCAGGAAACTCCCATCTTGACTATCCCAAAGATTCCGA CTTGGTGGAAGCCTTTCACTGTGATAAGACCCAGGTCAACAGATACAAGCTGTCGCTACTGAAGCCCTCT ACAAAGGCACTAGCTTTATCCTGTAAAGTGTCTATCCGGACAGATAACCGAGGCTTCCTCTCCCTACAGT ACATGATTAGAAATGAAGATGGGCAGATATGTTTTGTGGAATATTACTGCTGCCCTGATGAAGAAGTTCC TGAGTCTTGAATAATTCACTGACTATTTCTGTCTTTTTGTTTTTTTTTTCTTTTTTCTTTTGCGAGACAG TTTTTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTAGACCAGGCTGGCCTCGAACTCAGAAATTCAC CTGCCTCTGCCTCCTGAGTGCTGGGATTAAAGGCGTGCGCCACCACGACCGGCGAATATTTGTGTCTTTA TAGTTGGCGTTCTCACTGTAACTCTCTAACCTTGATGATTTCATTTTGGATTGTATAGAGAGATGCATGG AGAAAATAGGAGCAAGGTCAGTCCCATTCTGTAAATCTGTAAATAGTTTTTATTTTGTCAATAAATTTTT CATATAGCCATAAATTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC048693
• Insert Size: 843 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC048693, AAH48693
• RefSeq Size: 1408 bp
• RefSeq ORF: 843 bp
• Locus ID: 19355
• Cytogenetics: 15 A1
• Gene Summary: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity (By similarity).[UniProtKB/Swiss-Prot Function]