Mlf1 (BC027526) Mouse Untagged Clone

CAT#: MC206562

Mlf1 (untagged) - Mouse myeloid leukemia factor 1 (cDNA clone MGC:41163 IMAGE:1495721), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mlf1
• Synonyms: HLS7
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC027526
  CAGGCTGGTGGCGGCGCAGAGAGCTGCGCCCATTTCCATCCGCGCCCGCCGCCCGCCGCAGGATCACGAT GTTCCGGATGCTGAGCAGCAGCTTTGAGGATGACCCCTTCTTCGCTGATTCTTTTCTTGCACACCGAGAA AGTATGCGCAACATGATGAGAAGTTTCTCTGAACCTCTTGGAAGAGACTTGCTCAGTATCTCTGATGGTA GAGGAAGAACCCATAATCGTCGAGAACGTGATGATGGCGAAGATTCCTTAACTGCAACAAGTTTTTCTCT TGTGCCTTTTGGAAGGTTTGGTGGAATGCATGCAGATGTCAACCCTTTTCAGACAATGGATCGGATGATG GCAAATATGCGAAGTGGTATACAGGAGTTACAAAGAAACTTTGGCCAACTTTCAATGGATCCAAATGGGC ATTCATTTTGTTCTTCCTCTGTTATGACCTATTCCAAAGTAGGAGATGAACCACCAAAGGTGTTCCAGGC CTCAACTCAAACCCGAAGGGCTCCAGGAGGAGTAAAAGAAACCAGAAAAGCAATGAGAGATTCTGACAGT GGACTAGAAAGAATGGCTGTTGGTCATCACATCCATGACCGAGGTCATGTCATTAGGAAGTCAAAGAACA ACAAGACTGGAGATGAAGAAGTCAACCAAGAGTTCATCAATATGAATGAAAGTGACGCTCATGCTTTTGA TGATGAGTGGCAAAATGAAGTTCTGAAGTACAAGTCTATTGGACGGTCAGGAAACACTGGAATGCGAAGT GTGGGTCATGAGCATCCAGGGTCACGGGAACTCAAAAGGAGAGAGAAAATTCATCGAAATTCAGCCATTG AGAGTGGAAGAAGATCAAACGTTTTTGTGGACAAACTCAATGTGAAAGGATCACCTGTGAAAATCACCAA AAAATAAATAGCCCTGCATTTCATTTGTTTAGTTGGTTATTTTAACAAAGTCATGGCATGGTGCTAGAAA ATACACAAAAGACCAATCTCCTGTTGTTAAATCAATGCTGTACTTTGCAACTTTCTTCCAAATATGAATG TTCTTGTAAGTTTTAGCTTTACATTGTTCATACTTTAGGAATAAAATATGGATGTTCAAAAAAAAAAAAA AAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC027526
• Insert Size: 849 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027526, AAH27526
• RefSeq Size: 1126 bp
• RefSeq ORF: 849 bp
• Locus ID: 17349
• Cytogenetics: 3 30.91 cM
• Gene Summary: Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.[UniProtKB/Swiss-Prot Function]