Atp1a3 (BC020177) Mouse Untagged Clone

CAT#: MC206520

Atp1a3 (untagged) - Mouse ATPase, Na+/K+ transporting, alpha 3 polypeptide (cDNA clone MGC:27631 IMAGE:4506376), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp1a3
• Synonyms: MGC27631, MGC38713, MGC38914, MGC39036
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC020177
  CCCACGGACCGACAGACGCACGCTCCCACCGCGGCGCGGGCGCTGCAGAGGCCCCCAGCCCGAGCCCGCG CCTGAGCCCATCCTGCGGCCACCGCTCATCAGTCTGAACGCCGCTCTTCCCGCGGAGCCGCCAAGATGGG GGACAAAAAAGATGACAAGAGCTCGCCCAAGAAGAGCAAGGCCAAAGAGCGCCGGGACCTGGATGACCTC AAGAAGGAAGTGGCTATGACAGAGCACAAGATGTCAGTAGAAGAGGTCTGCCGGAAATACAATACTGACT GCGTGCAGGGTCTGACACACAGTAAAGCCCAGGAGATCCTAGCCCGGGATGGGCCTAACGCCCTCACACC ACCGCCCACCACCCCAGAATGGGTCAAGTTCTGCCGGCAGCTGTTTGGTGGCTTCTCTATCCTGCTGTGG ATCGGGGCAATCCTTTGCTTCCTGGCCTATGGCATCCAGGCAGGGACGGAGGATGACCCTTCCGGTGACA ATCTGTACCTGGGCATAGTGCTGGCCGCAGTCGTGATCATCACCGGCTGCTTCTCCTACTACCAAGAAGC CAAGAGTTCTAAGATCATGGAGTCCTTCAAGAACATGGTCCCCCAGCAAGCCCTTGTGATCCGGGAAGGT GAAAAGATGCAGGTGAATGCGGAGGAGGTGGTGGTCGGGGACCTGGTGGAGATCAAGGGTGGTGACCGGG TGCCAGCTGACCTGCGCATCATCTCGGCCCATGGCTGCAAGGTGGACAACTCCTCCCTGACTGGCGAATC TGAGCCTCAGACCCGCTCCCCGGACTGCACACACGACAACCCCCTGGAGACTCGGAACATCACCTTCTTT TCCACCAACTGCGTGGAAGGCACCGCTCGGGGTGTGGTGGTAGCCACAGGTGACCGCACCGTCATGGGCC GCATTGCCACCCTGGCCTCGGGCTTGGAGGTGGGCAAGACGCCCATCGCCATTGAGATTGAGCATTTCAT CCAGCTCATTACGGGCGTGGCCGTGTTCCTGGGCGTGTCCTTCTTCATCCTCTCTCTCATTCTGGGTTAC ACCTGGCTCGAGGCAGTCATCTTCCTCATCGGCATCATTGTGGCCAATGTCCCAGAGGGGCTGCTGGCTA CTGTCACGGTGTGTCTGACGCTGACCGCCAAGCGCATGGCTCGCAAGAACTGCCTGGTGAAGAACCTGGA GGCGGTGGAGACGCTGGGCTCCACGTCCACCATCTGCTCGGACAAGACCGGCACTCTCACCCAGAACCGC ATGACCGTCGCCCACATGTGGTTTGACAACCAGATCCACGAGGCAGACACCACAGAGGATCAGTCAGGGA CCTCTTTCGACAAGAGCTCACACACCTGGGTGGCCCTGTCCCACATCGCCGGGCTCTGCAACCGGGCCGT CTTCAAGGGCGGGCAGGACAACATCCCAGTACTCAAGAGGGACGTGGCCGGTGATGCCTCCGAGTCTGCC CTGCTTAAGTGCATTGAGCTGTCCTCGGGTTCCGTAAAGCTGATGCGAGAACGGAACAAGAAAGTGGCTG AGATTCCGTTCAACTCGACCAACAAATACCAGCTATCCATCCATGAGACTGAGGATCCCAATGACAACCG GTACCTGCTAGTGATGAAGGGCGCCCCCGAACGCATTCTGGACCGCTGTGCCACCATCCTCCTGCAGGGC AAGGAGCAGCCTCTGGATGAGGAGATGAAGGAGGCCTTCCAGAATGCCTACCTGGAGCTTGGTGGCCTGG GCGAGCGTGTGCTGGGTTTCTGCCATTACTACCTGCCTGAGGAACAGTTCCCCAAGGGCTTTGCCTTTGA CTGTGATGACGTGAACTTCACCACAGACAACCTTTGCTTTGTGGGTCTCATGTCCATGATTGACCCTCCC CGGGCAGCCGTCCCTGACGCTGTGGGCAAATGCCGAAGCGCAGGCATCAAGGTCATCATGGTCACCGGCG ATCACCCCATCACTGCCAAGGCCATTGCCAAGGGTGTGGGTATCATCTCTGAGGGTAACGAGACTGTCGA AGACATCGCTGCCCGGCTCAACATCCCTGTCAGCCAGGTGAACCCCAGGGATGCCAAAGCCTGTGTGATT CACGGCACCGACCTCAAGGACTTCACCTCGGAGCAGATTGACGAGATTCTGCAGAACCACACCGAGATCG TCTTTGCCCGAACCTCCCCTCAGCAGAAGCTCATCATCGTGGAGGGCTGTCAGAGACAGGGAGCAATTGT GGCTGTGACTGGCGATGGTGTGAATGACTCCCCTGCTCTGAAGAAGGCTGACATCGGGGTGGCCATGGGC ATTGCTGGCTCTGATGTCTCTAAGCAGGCTGCCGACATGATTCTGCTGGATGACAACTTTGCTTCCATTG TCACTGGTGTGGAGGAAGGCCGCCTGATCTTTGACAACCTGAAGAAATCCATCGCCTACACTCTGACTAG CAACATCCCTGAGATCACACCCTTCCTGCTCTTCATCATGGCTAACATCCCACTGCCTCTTGGCACCATC ACCATCCTCTGCATTGACCTGGGTACCGACATGGTCCCTGCAATCTCCCTGGCCTACGAGGCTGCCGAGA GCGACATCATGAAGAGGCAGCCCAGGAACCCACGCACAGACAAACTGGTCAACGAAAGGCTCATCAGCAT GGCCTACGGGCAGATTGGGATGATCCAGGCCCTCGGTGGTTTCTTCTCCTACTTTGTCATCCTGGCAGAA AATGGCTTCTTGCCCGGAAACCTGGTGGGCATCCGGCTCAACTGGGATGATCGCACTGTCAATGACCTAG AAGACAGTTATGGGCAGCAGTGGACTTATGAGCAGAGGAAGGTGGTAGAGTTCACATGCCACACAGCCTT CTTTGTGAGTATCGTGGTGGTCCAGTGGGCTGACCTGATCATCTGCAAGACCAGGAGGAACTCCGTCTTC CAGCAGGGCATGAAGAATAAGATCTTGATCTTCGGCTTGTTTGAGGAGACGGCCCTCGCTGCCTTCCTGT CCTACTGCCCAGGCATGGATGTGGCCCTTCGCATGTACCCTCTCAAGCCCAGCTGGTGGTTCTGTGCCTT CCCCTACAGTTTCCTCATCTTCGTCTATGATGAGATTCGCAAACTCATCCTGCGCAGGAACCCCGGGGGT GAGGGCCGCATGGCCAGGGGTAAAGGCTGGGAGGGGAAGCAGAGGGTGAGCACTCCCTGTGCCTCTCAGA TGTGTCCGTTCTTCTTTCTCTTCCCTCTCTCCCCGCCTTGCCTTTCACTCTCTTTCCAAGTGCGACTGTG TGTCTGAAAATAGGTTCTCCTTATGTAGCTCAGGCTGCCCTTGAGCTCATGTCCCTGAGTTTCTCAGTGC TGGGATGACAGGCCTGCCACCACACCTTGTGTTTGTGTCAATGTTTCTTTCTGTGTCCCTGTCTCTTGCT CATATGGCTCTCGTGTCTGCCTTCCTCTCACTTTGACTCTCTTCTGTCATTCCAGGTTGGGTGGAGAAAG AGACCTACTATTGACCTCAGCCACCACCACGTCGCCCGTGTCTTCCCTACCCCAAGCCCAGGACAGCCCT CTCCGGTCCCCCCATTTTGTACTCTGGGGGGAGGGGCCTTCTCTCCCCATGGCCCCCCTCTCTCCTTGTG GCCCAGCCTCCTCTCCCACACCTCGATTACCTCCCACGTTCCCTCACTTCTTCCTCTGGCTGGCTTCTCT CCCCCCACAGCCCCTGCCTCTCTTCTCCCTCTCTCTTCTGTGTCTCTCCCCCTCTCTCCCTTGCAACTCT GCCTCTCATTTCCTCCCTGGGCTCTTTTTTACTCCCCTGGGCCCCGTCTCCTGGCGTATGCCATCTCCGG TTCTAATTCTGAACAATTATCAAATATATCAGTGGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC020177
• Insert Size: 3162 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020177, AAH20177
• RefSeq Size: 4043 bp
• RefSeq ORF: 3162 bp
• Locus ID: 232975
• Cytogenetics: 7 13.73 cM
• Gene Summary: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity).[UniProtKB/Swiss-Prot Function]