Rapgef3 (BC020532) Mouse Untagged Clone

CAT#: MC206515

Rapgef3 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (cDNA clone MGC:19192 IMAGE:4236136), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rapgef3
• Synonyms: Epac, MGC19192, Epac1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC020532, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGTTGAAGAGAATGCACCGTCCCCGCTGCTGCTCTTACCAGCTAGTGTTCGAGCACCGGCGCCCCA
  GCTGCATCCAGGGACTCCGCTGGACACCACTTACCAACAGCGAGGACTCCCTGGATTTCAGAGTGAGTCT
  GGAGCAGGCCACCACAGAGCATGTGCACAAGGCAGGGAAGCTCCTGCACCGCCATCTCTTGGCCACGTAC
  CCTACCCTCATCCGAGACAGAAAATACCATCTGCGACTATATCGGCATTGCTGCTCTGGCCGGGAGCTAG
  TGGATGGGATCTTGGCTCTGGGGCTTGGGGTCCACTCACGGAGCCAAGCCGTGGGCATCTGCCAAGTGTT
  GCTGGATGAGGGTGCCCTTTGTCATGTAAAACATGACTGGACCTTCCAGGACCGAGATGCCCAATTCTAC
  AGGTTCCCTGGACCGGAGCCCGAGCCTACAGGAACTCAAGATGTGGAAGAGGAGCTTGTTGAGGCTATGG
  CCCTCCTGTCCCAGCGAGGGCCTGATGCCCTACTCACCGTGGCGCTCCGGAAGCCCCCAGGTCAGCGTAC
  GGATGAAGAACTGGACCTGATCTTTGAAGAGCTGCTGCATATCAAGGCGGTGGCACACCTTTCTAACTCG
  GTGAAGCGGGAACTAGCTGCTGTTCTGCTCTTTGAACCACACAGCAAGGCAGGAACTGTGTTGTTCAGCC
  AGGGGGACAAGGGTACCTCGTGGTACATTATCTGGAAGGGATCTGTCAATGTGGTGACCCATGGCAAGGG
  GCTGGTGACCACGTTGCACGAGGGAGATGACTTTGGACAGCTGGCTCTGGTGAACGACGCACCTCGGGCA
  GCCACCATCATCCTTCGAGAAAATAACTGTCACTTTCTGCGTGTGGACAAGCAGGACTTCAACCGCATCA
  TCAAGGATGTGGAAGCAAAAACCATGAGACTGGAAGAACACGGCAAAGTGGTCTTAGTTCTGGAGAGAAC
  CTCTCAGGGTGCTGGCCCTTCCCGTCCCCCGACCCCAGGCAGGAACCGGTATACGGTCATGTCTGGCACC
  CCAGAGAAAATCCTAGAACTGCTGTTGGAGGCTATGAGACCGGATTCCAGTGCTCATGACCCAACAGAGA
  CGTTCCTCAGTGACTTCCTGCTGACCCACAGTGTCTTCATGCCCAGCACCCAGCTCTTCACTGCCCTCCT
  GCACCACTTCCACGTGGAGCCAGCAGACCCTGCTGGAGGCAGCGAGCAGGAGCACAGCACCTACATCTGC
  AACAAGAGGCAGCAGATCCTGCGGCTAGTTGGCCGATGGGTGGCCCTGTATAGCCCGATGCTCCACTCGG
  ATCCCGTGGCCACCAGCTTCCTCCAGAAACTCTCAGACCTGGTGAGCAGAGATGCCCGACTTAGCAACTT
  GCTGAGGGAACAGTATCCAGAGAGACGGCGACACCACAGGTTGGAAAATGGCTGTGGGAACGTATCTCCT
  CAGACCAAGGCCCGGAATGCACCTGTTTGGCTCCCTAACCAGGAGGAACCCCTCCCAAGCAGCGCGGGTG
  CCATCCGAGTTGGGGACAAAGTCCCCTACGACATCTGCAGACCTGACCACTCAGTGCTGACCCTGCACCT
  GCCGGTGACGGCCTCGGTGAGAGAAGTGATGGCAGCTTTGGCCCATGAGGACCACTGGACCAAGGGACAG
  GTGTTGGTGAAGGTCAATTCTGCCGGTGATGTCGTTGGCTTGCAGCCAGATGCCCGTGGTGTGGCCACAT
  CCCTGGGGCTCAACGAGCGGCTCTTTGTTGTCGACCCACAGGAAGTGCATGAGCTGACCCCACACCCTGA
  GCAGCTGGGCCCCACTCTGGGTTCTTCTGAGATGCTGGACCTAGTGAGCGCCAAGGACCTGGCAGGCCAG
  CTGACCGACCATGACTGGAACCTCTTCAACAGGATCCACCAGGTGCAGGAGCACCTGCGGGATGTCACCA
  CCGCGAACCTGGAGCGCTTCATGCGCCGCTTCAACGAGCTGCAGTACTGGGTGGCCACAGAGCTCTGTCT
  CTGCCCTGTTCCTGGCTCCCGGGCTCAGCTACTCAGGAAGTTCATCAAGCTGGCAGCCCACCTCAAGGAG
  CAGAAGAACCTCAACTCTTTCTTTGCGGTCATGTTTGGCCTCAGCAACTCGGCCATCAGCCGGCTGGCCC
  ACACCTGGGAGCGCCTGCCCCATAAAGTACGGAAGCTGTACTCAGCCCTGGAAAGGTTGCTGGACCCTTC
  CTGGAACCACCGAGTGTACCGATTGGCTCTCACCAAGCTCTCCCCTCCTGTCATCCCCTTCATGCCCCTG
  CTGCTCAAAGACGTGACCTTCATCCATGAGGGGAACCACACACTGGTAGAGAACCTCATCAACTTTGAGA
  AGATGCGGATGATGGCCAGAGCGGTGCGGATGCTTCACCACTGCCGAAGTCACAGTACCGCGCCTCTATC
  ACCACTCAGAAGCCGAGTGTCCCACATCCACGAGGACAGCCAGGGATCAAGGATCTCCACGTGTTCTGAG
  CAGTCCCTGAGCACCCGGAGTCCAGCCAGCACCTGGGCTTATGTCCAGCAGCTGAAGGTCATTGACAACC
  AGCGGGAACTGTCCCGCCTCTCCCGGGAGCTGGAACCATGA
  
  
• Restriction Sites: EcoRI-NotI
• ACCN: BC020532
• Insert Size: 2631 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020532, AAH20532
• RefSeq Size: 3644 bp
• RefSeq ORF: 2631 bp
• Locus ID: 223864
• Cytogenetics: 15 F1
• Gene Summary: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin (By similarity).[UniProtKB/Swiss-Prot Function]