AU018778 (BC013479) Mouse Untagged Clone

CAT#: MC206510

AU018778 (untagged) - Mouse expressed sequence AU018778 (cDNA clone MGC:18894 IMAGE:4239756), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: AU018778
• Synonyms: MGC18894
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC013479
  GTCCATGCAAGATGTTCCTTAGCACTCTGTTCCTGGTGTCTCTAGCAACCTGTGTGATTTGCGGAAATCC CTCTTCACCACCTGTGGTAGACACTGCTCATGGTAAAGTCCTGGGGAAACACGTGAACGTAGAAGGATTT TCACAGCCTGTGGCCGTCTTCCTGGGAATCCCCTTTGCCAAGCCCCCTCTTGGATCCCTGAGGTTTGCTC CACCACAGCCTGCAGAGCCCTGGAGCTCAGTGAAGAATGCCACCACCTACCCACCTATGTGCTCCCAAGA TGCAGCTAGAGGACAGGCGGTCAATGACCTCATAACCAATAGAAAGGAGAAAATCCATCTTGAATTTTCT GAAGATTGCCTGTACCTAAATATTTACACTCCTGCGGACTTTTCAAAGAACAGTAGGCTACCGGTGATGG TGTGGATCCATGGAGGTGGACTGAAGCTGGGTGGGGCATCAAGCTTTGATGGACGGGCTCTCTCTGCATA CGAAAATGTGGTGGTGGTGGCCATTCAATATCGCCTGAGTATCTGGGGATTCTTCAGCACAGGGGATGAA CACAGTCGGGGAAACTGGGGTCATTTGGACCAAGTGGCTGCTCTGCATTGGGTCCAGGACAACATTGCCA ACTTTGGCGGGGACCCAGGCTCTGTGACCATCTTTGGAGAGTCAGCAGGAGGTTACAGTGTCTCAATTCT TATATTGTCCCCATTGTCCAAGAACCTCTTCCACAGGGCCATTTCTGAGAGTGGTGTGGCCTTCATTCCT GGAATGTTTACCAAAGATGTGAGGCCAATTACTGAGCAAATTGCTGTTACTGCTGGCTGTAAGACCACCA CGTCTGCCGTCATTGTTCACTGCATGCGCCAGAAGACGGAGGAGGAGCTATTAGAGATCATGCATAAATT GAATCTGTATAAACTGAGTTTACAAGGAGATACCAAAAATAGCGACCAGTTCGTGACAAGTGTGCTTGAT GGAGTGGTGCTACCAAAGGACCCCAAAGAGATCCTGGCTGAGAAGAACTTCAACACTGTGCCTTACATTG TGGGAATCAACAAGCAAGAATGTGGCTGGCTTCTGCCAACAATGACGGGATTTCTACCAGCTGATGTAAA ATTGGACAAGAAGAAAGCCATTGCACTCCTGGAGCAATTTGCTTCCATGACTGGCATACCAGAGGATATT ATTCCAGTTGCTGTTGAGAAGTACACAAAAGGTAGTGATGACCCTGATCAGATCAGAGAGGGAGTTCTCG ACGCAATGGGGGATGTGGCATTTGGTGTTCCATCGGTGATTGTGTCCCGTGGCCACAGAGACACTGGAGC TCCCACCTACATGTATGAGTATCAATACTACCCAAGCTTCTCATCACCCCAAAGACCCAAGAATGTAGTA GGAGACCATGCAGATGATGTCTACTCTGTCTTCGGTGCTCCAATTTTAAGAGAGGGTGCCTCCGAAGAGG AGATCAATCTCAGCAAGATGGTGATGAAATTCTGGGCCAACTTTGCTCGGAATGGGAACCCTAATGGCAA AGGGCTGCCTCATTGGCCAAAGTATGATCAGAAAGAAGGATATCTTCATATTGGTGGCACCACCCAGCAA GCCCAGAGACTGAAGGAAGAGGAAGTGACTTTCTGGACACAGTCCCTTGCCAAGAAACAACCCCAGCCAT ACCACAATGAGCTGTGAATGCAAAGCTCTGCCAGGCTTATACCAGAGAAGCCAAGATATGGTTCTTCCAC TAACAGTGTATGTAAATCCAAGATGGATCTTGGAGGATCCTGAAGAACTTTGTCATAGATATAGGCAGAG CCCAGGGAAGGGAAATGTTGTATTTATGTCTTTAATTTAGAGAATAAATGTTCTTTATATGGTCAAAAAA AAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC013479
• Insert Size: 1686 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013479, AAH13479
• RefSeq Size: 1909 bp
• RefSeq ORF: 1686 bp
• Locus ID: 234564
• Cytogenetics: 8 C5
• Gene Summary: Hydrolyzes p-nitrophenyl butyrate (PNPB), triacylglycerol and monoacylglycerol. Shows higher activity against PNPB, a short-chain fatty acid ester, than against triolein, a long-chain fatty acid ester. Shows no detectable activity against diacylglycerol, cholesterol ester or phospholipids. May play a role in adipocyte lipolysis.[UniProtKB/Swiss-Prot Function]